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首页> 外文期刊>Cell cycle >Clusters, factories and domains: The complex structure of S-phase comes into focus.
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Clusters, factories and domains: The complex structure of S-phase comes into focus.

机译：集群，工厂和域：S期的复杂结构成为焦点。

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摘要

During S-phase of the cell cycle, chromosomal DNA is replicated according to a complex replication timing program, with megabase-sized domains replicating at different times. DNA fibre analysis reveals that clusters of adjacent replication origins fire near-synchronously. Analysis of replicating cells by light microscopy shows that DNA synthesis occurs in discrete foci or factories. The relationship between timing domains, origin clusters and replication foci is currently unclear. Recent work, using a hybrid Xenopus/hamster replication system, has shown that when CDK levels are manipulated during S-phase the activation of replication factories can be uncoupled from progression through the replication timing program. Here, we use data from this hybrid system to investigate potential relationships between timing domains, origin clusters and replication foci. We suggest that each timing domain typically comprises several replicon clusters, which are usually processed sequentially by replication factories. We discuss how replication might be regulated at different levels to create this complex organisation and the potential involvement of CDKs in this process.
机译：在细胞周期的S期，根据复杂的复制定时程序复制染色体DNA，并在不同时间复制兆碱基大小的域。 DNA纤维分析表明，相邻复制起点的簇几乎同步激发。通过光学显微镜对复制细胞的分析表明，DNA合成发生在离散的病灶或工厂中。时序域，原始群集和复制焦点之间的关系目前尚不清楚。最近使用混合非洲爪哇/仓鼠复制系统的工作表明，在S阶段操作CDK级别时，复制工厂的激活可以通过复制定时程序与进程脱钩。在这里，我们使用来自该混合系统的数据来调查时序域，原始群集和复制焦点之间的潜在关系。我们建议每个时序域通常包含几个复制子簇，这些簇通常由复制工厂顺序处理。我们讨论如何在不同级别上对复制进行监管以创建这个复杂的组织，以及CDK在此过程中的潜在参与。

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