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首页> 外文期刊>Cellular Physiology and Biochemistry >The Squid Axon Na+/Ca2+ Exchanger Shows Ping Pong Kinetics only when the Ca-i-regulatory Site is Saturated
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The Squid Axon Na+/Ca2+ Exchanger Shows Ping Pong Kinetics only when the Ca-i-regulatory Site is Saturated

机译：鱿鱼轴突Na + / Ca2 +交换子仅在Ca-i调节位点饱和时显示乒乓动力学

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摘要

In a previous work we demonstrated that, in dialyzed squid axons, an impairment of the Ca-i(2+)-regulatory site affected the apparent affinities for external Na+ and Ca2+ in a way opposite to that predicted by the exiting (ping-pong) models for the exchangers. In the present work, we used model simulations and actual experiments where the Ca-i(2+)-regulatory remained always saturated while [Ca2+](i) was either limiting or near saturating for the internal Ca2+ transport sites. Under these conditions, both the theoretical and experimental transport activation curves for external Na+ and Ca2+ were those expected from the current kinetic schemes. These observations have two important implications: On the one hand, they confirm the ping-pong translocation schemes for Na+/Ca2+ exchange. On the other, they call for caution in interpreting kinetic data in membrane transport systems possessing intracellular ionic and/or metabolic regulation.
机译：在以前的工作中，我们证明了，在渗碳鱿鱼轴突中，Ca-i（2+）调控位点的损伤会影响外部Na +和Ca2 +的表观亲和力，其方式与出口预测的相反（乒乓球） ）的模型。在目前的工作中，我们使用模型模拟和实际实验，其中Ca-i（2+）的调节作用始终保持饱和，而内部Ca2 +转运位点的[Ca2 +]（i）则为极限或接近饱和。在这些条件下，外部Na +和Ca2 +的理论和实验输运活化曲线都是当前动力学方案所预期的。这些观察有两个重要的含义：一方面，他们证实了Na + / Ca2 +交换的乒乓易位方案。另一方面，他们在解释具有细胞内离子和/或代谢调节作用的膜转运系统中的动力学数据时需要谨慎。

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