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首页> 外文期刊>Cell cycle >MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation.
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MEK1/2 regulates microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte meiotic maturation.

机译：MEK1 / 2调节小鼠卵母细胞减数分裂成熟过程中的微管组织，纺锤体极束缚和不对称分裂。

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It is well known that MAPK plays pivotal roles in oocyte maturation, but the function of MEK (MAPK kinase) remains unknown. We have studied the expression, subcellular localization and functional roles of MEK during meiotic maturation of mouse oocytes. Firstly, we found that MEK1/2 phoshorylation (p-MEK1/2, indicative of MEK activation) was low in GV (germinal vesicle) stage, increased 2h after GVBD (germinal vesicle breakdown), and reached the maximum at metaphase II. Secondly, we found that P-MEK1/2 was restricted in the GV prior to GVBD. In prometaphase I and metaphase I, P-MEK1/2 was mainly associated with the spindle, especially with the spindle poles. At anaphase I and telophase I, p-MEK1/2 became diffusely distributed in the region between the separating chromosomes, and then became associated with the midbody. The association of p-MEK1/2 with spindle poles was further confirmed by its colocalization with the centrosomal proteins, gamma-tubulin and NuMA. Thirdly, we have investigated the possible functional role of MEK1/2 activation by intravenous administration and intrabursal injection of a specific MEK inhibitor, U0126, and by microinjection of MEK siRNA into oocytes. All these manipulations cause disorganized spindle poles and spindle structure, misaligned chromosomes and larger than normal polar bodies. Our results suggest that MEK1/2 may function as a centrosomal protein and may have roles in microtubule organization, spindle pole tethering and asymmetric division during mouse oocyte maturation.
机译：众所周知，MAPK在卵母细胞成熟中起关键作用，但MEK（MAPK激酶）的功能仍然未知。我们已经研究了MEK在小鼠卵母细胞减数分裂成熟过程中的表达，亚细胞定位和功能作用。首先，我们发现MEK1 / 2磷酸化（p-MEK1 / 2，指示MEK活化）在GV（胚泡）阶段低，在GVBD（胚泡破裂）后2小时增加，并在中期II达到最大值。其次，我们发现P-MEK1 / 2在GVBD之前在GV中受到限制。在前期I和中期I中，P-MEK1 / 2主要与纺锤体相关，特别是与纺锤体极相关。在后期I和末期I，p-MEK1 / 2散布在分离染色体之间的区域中，然后与中体缔合。 p-MEK1 / 2与纺锤体极的缔合通过其与中心体蛋白，γ-微管蛋白和NuMA的共定位进一步得到证实。第三，我们研究了通过静脉内施用和囊内注射特定MEK抑制剂U0126以及通过将MEK siRNA显微注射到卵母细胞中MEK1 / 2激活的可能功能作用。所有这些操作会导致纺锤体极轴和纺锤体结构混乱，染色体错位并且比正常极体大。我们的结果表明，MEK1 / 2可能起着中心体蛋白的作用，并且可能在小鼠卵母细胞成熟过程中在微管组织，纺锤体极束缚和不对称分裂中起作用。

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