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首页> 外文期刊>Research on Crops >Agro-morphological variation among two selected wheat varieties after ethylmethanesulphonate mutagenesis
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Agro-morphological variation among two selected wheat varieties after ethylmethanesulphonate mutagenesis

机译：甲基磺酸乙酯诱变后两个选定小麦品种的农业形态变化

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The study was aimed at investigating variations in agro-morphological traits of two selected wheat varieties after chemical mutagenesis using ethylmethanesulphonate (EMS). Two varieties (SST 56 and SST 875) were subjected to EMS mutagenesis using 0.5%v/v EMS at 32.5°C for 1 h. Field trials were carried out at Ukulinga Research Farm of the University of KwaZulu-Natal using a randomized complete block design with two replications. Data on nine important agro-morphological traits were collected and analyzed using the analysis of variance (ANOVA), correlation and principal component analysis (PCA) procedures. Significant variations were found among the agro-morphological traits between M, individuals of the varieties after the mutagenesis compared tountreated checks. EMS significantly reduced seed germination in the field at 40% in both the varieties and significantly delayed days to heading by eight days. The mutagen caused a shortening of the days to maturity by 13 days in both the varieties. EMStreatment also significantly reduced plant height tol8 and 21cm in SST 56 and SST 875, respectively. The spike length was also reduced by -2.5 cm in both the varieties compared to control. 100-seed weight, flag leaf length and seeds per spike significantly increased in the treated seeds of both the varieties. Plant height had positive and significant correlation with number of tillers, number of seeds per spike, flag leaf length and 100-seed weight. However, it had negative correlation with the number of days to maturity. PCA revealed that three principal components (PC 1, PC 2 and PC 3) accounted to 57% of the total variations among the agro-morphological traits in both the varieties. PC 1 alone contributed to 27.7% of the variation which was correlated well with plant height (0.767), tiller number (0.812), number of seeds per spike (0.599) and seed yield (0.720). PC 2 explained 15.6% of the variation and well-correlated with germination percentage (0.784), spike length (0.554) and flag leaf length (0.772). PC 3 accounted to 12.4% of the variation and had negative correlation with days to maturity (-0.730). The study found that EMS had the potential to increase agro-morphological variations in wheat. This could be useful in selecting novel mutants with desired phenotypic traits.
机译：该研究旨在调查使用甲基磺酸乙酯（EMS）进行化学诱变后，两个选定小麦品种的农业形态性状变异。使用0.5％v / v EMS在32.5°C下对两个变种（SST 56和SST 875）进行EMS诱变1小时。夸祖鲁-纳塔尔大学（University of KwaZulu-Natal）的Ukulinga研究场进行了田间试验，使用了随机的完全区组设计，重复两次。收集并使用方差分析（ANOVA），相关性和主成分分析（PCA）程序分析了9个重要的农业形态性状的数据。与未经处理的检查相比，诱变后的M，该品种个体之间的农业形态性状之间发现了显着差异。在两个品种中，EMS均显着降低了田间种子发芽率，均为40％，并且将抽穗的天数显着延迟了八天。诱变剂使两个品种的成熟期缩短了13天。 EMS处理还显着降低了SST 56和SST 875的株高分别为18和21厘米。与对照相比，两个品种的穗长也减少了-2.5 cm。两个品种的处理种子中100粒重，旗叶长度和每个穗的种子显着增加。株高与分till数，每个穗的种子数，旗叶长度和100粒重有正相关和显着相关。但是，它与到期天数负相关。 PCA显示三个主要成分（PC 1，PC 2和PC 3）占两个品种农业形态性状总变异的57％。仅PC 1贡献了27.7％的变异，与植物高度（0.767），分till数（0.812），每个穗的种子数（0.599）和种子产量（0.720）密切相关。 PC 2解释了15.6％的变异，并且与发芽率（0.784），穗长（0.554）和旗叶长度（0.772）密切相关。 PC 3占变异的12.4％，与到期天数（-0.730）呈负相关。研究发现，EMS具有增加小麦农业形态变异的潜力。这可能对选择具有所需表型性状的新型突变体有用。

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