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首页> 外文期刊>Cell cycle >Annexin A5 is involved in migration and invasion of oral carcinoma.
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Annexin A5 is involved in migration and invasion of oral carcinoma.

机译：Annexin A5参与口腔癌的迁移和侵袭。

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Annexin A5 is a Ca(2+)-binding protein which is involved in membrane organization and dynamics. As recent data suggest a role of annexin A5 in cancer we aimed to gain more insight into the biological function of endogenous annexin A5 and assessed its possible influence on proliferation and invasion capacity. We downregulated annexin A5 by RNA interference in HaCaT keratinocytes, squamous carcinoma cell line A431 as well as in a primary cell culture of a human oral carcinoma. Hereby, we detected reduced migration and invasion capacity of HaCaT cells which was even stronger in the oral carcinoma. To determine target genes of annexin A5 we used a metastasis specific microarray. Thereby, genes implicated in cell motility including S100A4, TIMP-3 and RHOC were observed to be regulated. These deregulations were confirmed by RT-PCR or western blots, respectively. These observations suggest that the invasion capacity, a main characteristic of tumors, is at least partially regulated by annexin A5 in oral carcinoma.
机译：Annexin A5是一种Ca（2+）结合蛋白，参与膜的组织和动力学。由于最新数据表明膜联蛋白A5在癌症中的作用，我们旨在深入了解内源性膜联蛋白A5的生物学功能，并评估其对增殖和侵袭能力的可能影响。我们在HaCaT角质形成细胞，鳞状癌细胞系A431以及人类口腔癌的原代细胞培养物中通过RNA干扰下调膜联蛋白A5。因此，我们检测到HaCaT细胞的迁移和侵袭能力降低，这在口腔癌中更为明显。为了确定膜联蛋白A5的靶基因，我们使用了转移特异性微阵列。由此，观察到与细胞运动有关的基因包括S100A4，TIMP-3和RHOC被调节。这些失调分别通过RT-PCR或蛋白质印迹证实。这些观察结果表明，侵袭能力是肿瘤的主要特征，在口腔癌中至少部分受到膜联蛋白A5的调节。

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