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首页> 外文期刊>Cellular Physiology and Biochemistry >Dual Response of Human Leukemia U937 Cells to Hypertonic Shrinkage: Initial Regulatory Volume Increase (RVI) and Delayed Apoptotic Volume Decrease (AVD)
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Dual Response of Human Leukemia U937 Cells to Hypertonic Shrinkage: Initial Regulatory Volume Increase (RVI) and Delayed Apoptotic Volume Decrease (AVD)

机译：人类白血病U937细胞对高渗收缩的双重反应：初始调节体积增加（RVI）和延迟的细胞凋亡减少（AVD）

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Background/Aims: Osmotic cell shrinkage is a powerful trigger of suicidal cell death or apoptosis, which is paralleled and enforced by apoptotic volume decrease (AVD). Cells counteract cell shrinkage by volume regulatory increase (RVI). The present study explored the response of human U937 cells to hypertonic solution thus elucidating the relationship between RVI and AVD. Methods: Cell water, concentration of monovalent ions and the appearance of apoptotic markers were followed for 0.5-4 h after the cells were transferred to a hypertonic medium. Intracellular water, K+, Na+, and CI" content, ouabain-sensitive and-resistant Rb~ influxes were determined by measurement of the cell buoyant density in Percoll density gradient, flame emission analysis and 36（CI） assay, respectively. Fluorescent microscopy of live cells stained by acridine orange and ethidium bromide was used to verify apoptosis. Results: After 2-4 h incubation in hypertonic media the cell population was split into light (L) and heavy (H) fractions. According to microscopy and analysis of monovalent ions the majority of cells in the L population were healthy, while the H fractions were enriched with apoptotic cells. The density of L cells was decreasing with time, while the density of H cells was increasing, thus reflecting the opposite effects of RVI and AVD. At the same time, some of the cells were shifting from L to H fractions, indicating that apoptosis was gradually extending to cells that were previously displaying normal RVI. Conclusion: The findings suggest that apoptosis can develop in cells capable of RVI.
机译：背景/目的：渗透性细胞收缩是自杀性细胞死亡或凋亡的有力触发因素，这与凋亡体积减少（AVD）并存并增强。细胞通过体积调节增加（RVI）抵消细胞的收缩。本研究探索了人类U937细胞对高渗溶液的反应，从而阐明了RVI和AVD之间的关系。方法：将细胞转移至高渗培养基中0.5〜4 h，观察细胞水，单价离子浓度和凋亡标记物的出现。通过Percoll密度梯度，火焰发射分析和36（CI）测定法分别测量细胞浮力密度，从而测定细胞内水，K +，Na +和CI“含量，哇巴因敏感性和耐药性Rb〜内流。结果：在高渗培养基中孵育2-4小时后，将细胞群分为轻（L）和重（H）级分，并通过显微镜检查和单价分析离子表明，L群体中的大多数细胞是健康的，而H组分中富含凋亡细胞，L细胞的密度随时间下降，而H细胞的密度却在增加，这反映了RVI和AVD的相反作用同时，一些细胞正在从L级转移到H级，这表明细胞凋亡逐渐扩展到先前显示正常RVI的细胞。帽细胞凋亡可以在具有RVI能力的细胞中发展。

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