• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cellular Physiology and Biochemistry >High ambient glucose levels modulates the production of MMP-9 and alpha 5(IV) collagen by cultured podocytes
【24h】

High ambient glucose levels modulates the production of MMP-9 and alpha 5(IV) collagen by cultured podocytes

机译：高环境葡萄糖水平可调节培养的足细胞产生MMP-9和α5（IV）胶原的能力

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent evidences have demonstrated an important role for glomerular visceral epithelial cell ( podocyte) in the development and progression of diabetic nephropathy. We investigated the high-glucose (HG)-triggered signaling pathway and its role in matrix metalloproteinase (MMP) production in murine podocytes. The activity of 92-kDa (MMP-9) gelatinase, but not of 72 kDa (MMP-2), in an HG medium significantly increased during incubation of 2 to 3 days and decreased during incubation of more than 5 days revealed by Gelatin zymography. Opposite to the increases in MMP-9 activity, HG medium produced significant decreases in the protein levels of alpha 5(IV) collagen. Changes in MMP-9 activity were associated with the same pattern as MMP-9 mRNA levels in podocytes exposed to HG media. HG medium rapidly activated ERK1/2 MAPK in podocytes. Moreover, ERK1/2 activation was required for HG-induced enhancement of MMP-9 activity and a decrease in the level of a5( IV) collagen. HG incubation rapidly induced an increase in the nuclear accumulation of Ets-1 protein. Blocking the ERK pathway suppressed HG-induced expression and nuclear accumulation of transcriptional factor Ets-1, and MMP-9 mRNA expression. We suggest that short-or long-term exposure to HG concentrations increases or decreases MMP-9 production and alpha 5(IV) collagen expression in podocytes, this may contribute to the GBM abnormality caused by an imbalance in extracellular matrix (ECM) synthesis and degradation, and may play a critical role in the pathogenesis of proteinuria in diabetic nephropathy. Copyright (c) 2006 S. Karger AG, Basel.
机译：最近的证据表明，肾小球内脏上皮细胞（足细胞）在糖尿病性肾病的发生和发展中起着重要作用。我们调查了高葡萄糖（HG）触发的信号通路及其在鼠足细胞中基质金属蛋白酶（MMP）生产中的作用。明胶酶谱分析显示，在HG培养基中，培养2至3天时，92 kDa（MMP-9）明胶酶的活性显着提高，而在培养5天以上时，其72 kDa（MMP-2）的活性未显着升高。 。与MMP-9活性的增加相反，HG培养基产生的α5（IV）胶原蛋白水平显着下降。 MMP-9活性的变化与暴露于HG培养基的足细胞中MMP-9 mRNA水平的模式相同。 HG培养基可快速激活足细胞中的ERK1 / 2 MAPK。此外，ERK1 / 2激活是HG诱导的MMP-9活性增强和a5（IV）胶原蛋白水平降低所必需的。 HG孵育迅速诱导Ets-1蛋白的核积累增加。阻止ERK通路抑制HG诱导的转录因子Ets-1和MMP-9 mRNA表达以及核积累。我们建议，短期或长期暴露于HG浓度会增加或减少足细胞中MMP-9的产生和α5（IV）胶原的表达，这可能是由于细胞外基质（ECM）合成不平衡和降解，并可能在糖尿病性肾病蛋白尿的发病机理中起关键作用。版权所有（c）2006 S.Karger AG，巴塞尔。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号