Heat shock protein 90 recognized as an iron-binding protein associated with the plasma membrane of HeLa cells

Kovar J; Stybrova H; Novak P; Ehrlichova M; Truksa J; Koc M; Kriegerbeckova K; Scheiber-Mojdehkar B; Goldenberg H

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Heat shock protein 90 recognized as an iron-binding protein associated with the plasma membrane of HeLa cells

机译：热休克蛋白90被认为是与HeLa细胞质膜相关的铁结合蛋白

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Heat shock protein 90 (Hsp90) is a molecular chaperone abundant in eukaryotic cells. However, its exact role is not completely understood yet. Employing an iron-binding assay and mass spectrometric analysis, we have identified human Hsp90 as an iron-binding protein in membrane protein preparations of human HeLa cells. Western blot analysis and confocal microscopy confirmed that a portion of cellular Hsp90 is associated with the plasma membrane, but it does not seem to be expressed on the cell surface. The iron-binding assay with purified human Hsp90 confirmed iron binding by Hsp90. Thus we suggest that Hsp90 is an iron-binding protein associated with the plasma membrane. Copyright (C) 2004 S. Karger AG, Basel.

机译：热休克蛋白90（Hsp90）是在真核细胞中丰富的分子伴侣。但是，其确切作用尚不完全清楚。利用铁结合测定和质谱分析，我们已经鉴定了人Hsp90作为人HeLa细胞膜蛋白制剂中的铁结合蛋白。 Western印迹分析和共聚焦显微镜证实细胞Hsp90的一部分与质膜相关，但似乎未在细胞表面表达。用纯化的人Hsp90进行的铁结合测定证实了Hsp90对铁的结合。因此，我们认为Hsp90是与质膜相关的铁结合蛋白。版权所有（C）2004 S.Karger AG，巴塞尔。

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《Cellular Physiology and Biochemistry》 |2004年第2期|共6页
作者
Kovar J; Stybrova H; Novak P; Ehrlichova M; Truksa J; Koc M; Kriegerbeckova K; Scheiber-Mojdehkar B; Goldenberg H;
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正文语种 eng
中图分类细胞生理学;
关键词
heat shock protein 90; iron-binding protein; plasma membrane; HeLa cells; HEAT-SHOCK-PROTEIN; KINASE-ACTIVITY; TUMOR-CELLS; HSP90; TRANSFERRIN; OLIGOMERIZATION; PHOSPHORYLATION; INHIBITION; EXPRESSION; CDK4;

机译：热休克蛋白90;铁结合蛋白;质膜;HeLa细胞;热激蛋白;激酶活性;肿瘤细胞;HSP90;运铁蛋白;寡聚化;磷酸化;抑制;表达;CDK4;

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1. 蚕豆保卫细胞原生质体质膜ABA结合蛋白的理化特性 [J] . 王恒彬, 曹敏植物学报：英文版 . 1997,第001期
2. 蚕豆保卫细胞原生质体质膜ABA结合蛋白与气孔对ABA敏感性的关系 [J] . 王恒彬, 曹敏植物学报：英文版 . 1997,第002期
3. Heat shock protein 90 recognized as an iron-binding protein associated with the plasma membrane of HeLa cells [J] . Kovar J, Stybrova H, Novak P, Cellular Physiology and Biochemistry . 2004,第1a2期

机译：热休克蛋白90被认为是与HeLa细胞质膜相关的铁结合蛋白
4. Heat shock increases turnover of 90 kDa heat shock protein phosphate groups in HeLa cells [J] . Bensaude Olivier, Legagneux Vincent, Morange Michel FEBS Letters . 1991,第2期

机译：热休克可增加HeLa细胞中90 kDa热休克蛋白磷酸基团的转换
5. Electromagnetic fields at 2.45?￠????GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland Electromagnetic fields at 2.45?￠????GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland Electromagnetic fields at 2.45?￠????GHz trigger changes in heat shock proteins 90 and 70 without altering apoptotic activity in rat thyroid gland [J] . Francisco José Ares-Pena, Juan Antonio Rodríguez-González, José Manuel Leiro, Biology Open . 2012,第9期

机译：在2.45 ???? GHz的电磁场触发热休克蛋白90和70的变化而不会改变大鼠甲状腺的凋亡活性在2.45 ??????? GHz的电磁场触发热休克蛋白90和70的变化而不会改变大鼠甲状腺的凋亡活动在2.45 ??????? GHz的电磁场触发热休克蛋白90和70的变化，而不会改变大鼠甲状腺的凋亡活动
6. QUANTITATIVE ANALYZE PROTEINS DYNAMIC CHANGE FROM INTRACELLULAR MEMBRANE COMPARTMENTS TO PLASMA MEMBRANE OF L6 SKELETAL MUSCLE CELLS AFTER RAPID STIMULATION WITH INSULIN [C] . Xiulan CHEN, Ziyou CUI, Zhensheng XIE, The 12th International Beijing Conference and Exhibition on Instrumental Analysis : Conference Abstracts . 2007

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7. The expression of heat shock protein 70, heat shock protein 90 and heat shock factor 1 within the bovine midcycle corpus luteum after stimulation with temperature in vitro and PGF2alpha in vitro and in vivo. [D] . Kopka, Melissa N. 2006

机译：热激蛋白70，热激蛋白90和热激因子1在体外温度和体外及体内PGF2α刺激后在牛黄体中体中的表达。
8. Disruption of Heat Shock Protein 90 (Hsp90)-Protein Kinase Cδ (PKCδ) Interaction by (−)-Maackiain Suppresses Histamine H1 Receptor Gene Transcription in HeLa Cells [O] . Yuki Nariai, Hiroyuki Mizuguchi, Takeyasu Ogasawara, 2015

机译：（-）-Maackiain破坏热激蛋白90（Hsp90）-蛋白激酶Cδ（PKCδ）相互作用抑制HeLa细胞中组胺H1受体基因的转录
9. Heat shock increases turnover of 90 kDa heat shock protein phosphate groups in HeLa cells [O] . Legagneux Vincent, Morange Michel, Bensaude Olivier 1991

机译：热休克可增加HeLa细胞中90 kDa热休克蛋白磷酸基团的转换

Heat shock protein 90 recognized as an iron-binding protein associated with the plasma membrane of HeLa cells

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