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首页> 外文期刊>Cellular Physiology and Biochemistry >A novel non-neuronal hSK3 isoform with a dominant-negative effect on hSK3 currents
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A novel non-neuronal hSK3 isoform with a dominant-negative effect on hSK3 currents

机译：一种新型的非神经元hSK3亚型，对hSK3电流具有显性负作用

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摘要

We have identified a hSK3-transcript, hSK3_ex1c, which is generated by alternative splicing. Isoform hSK3_ex1c lacks the cytosolic N-terminus and the first transmembrane helix and is exclusively expressed in non-neuronal tissues. hSK3 transfected tsA cells showed a Ca2+-activated K+ current in patch-clamp experiments, whereas hSK3_ex1c transfected cells and cells co-transfected with both isoforms did not. We fused both isoforms to fluorescence proteins and observed hSK3 localization predominantly in the plasma membrane. The co-expression of hSK3 + hSK3_ex1c resulted in their cytoplasmic colocalization. Thus, hSK3_ex1c has a dominant-negative effect on hSK3 by preventing its transport into the plasma membrane. Copyright (C) 2004 S. Karger AG, Basel.
机译：我们已经确定了hSK3转录本hSK3_ex1c，它是由可变剪接产生的。异构体hSK3_ex1c缺少胞质N端和第一个跨膜螺旋，仅在非神经元组织中表达。在膜片钳实验中，hSK3转染的tsA细胞显示Ca2 +激活的K +电流，而hSK3_ex1c转染的细胞和用这两种同工型共转染的细胞则没有。我们将两种同工型融合到荧光蛋白上，并观察到hSK3主要位于质膜中。 hSK3 + hSK3_ex1c的共表达导致它们的胞质共定位。因此，hSK3_ex1c通过阻止hSK3转运进入质膜而对hSK3产生显性负作用。版权所有（C）2004 S.Karger AG，巴塞尔。

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