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首页> 外文期刊>Cellular Physiology and Biochemistry >Effect of lipid infusion on metabolism and force of rat skeletal muscles during intense contractions
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Effect of lipid infusion on metabolism and force of rat skeletal muscles during intense contractions

机译：强烈收缩期间输注脂质对大鼠骨骼肌代谢和力量的影响

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摘要

The hypothesis that during intense muscle contraction induced by electrical stimulation, long chain fatty acids (LCFA) might reduce mitochondrial ATP/ADP ratio, raising the contribution of glycolysis for ATP production was examined. The effect of a lipid infusion (Lipovenus emulsion) on UCP-3 mRNA level, lactate, glucose-6-phosphate (G-6P) and glycogen content was investigated in rat. Blood samples for determination of free fatty acids and lactate were collected at 0, 30 and 60 min during rest and at 0, 10 and 20 min during muscle contraction. The content of lactate, glycogen and G-6P was also determined in soleus (SO), red gastrocnemius (RG) and white gastrocnemius (WG) muscles collected immediately after muscle contraction period. In addition, the force level was determined during muscle contractions. The effect of Lipovenus emulsion on respiration of mitochondria isolated from rat skeletal muscle, and content of UCP-3 and lactate in cultured skeletal muscle cells was also determined. The in vivo experiments showed that Lipovenus induced a significant increase of UCP-3 mRNA levels. After Lipovenus infusion, lactate level was increased in RG muscle only, whereas the contents of glycogen and G-6P were decreased in both RG and WG muscles (P < 0.05). Lipovenus infusion failed to exert any effect on muscle force performance (P > 0.05). The in vitro experiments showed that Lipovenus infusion induced a significant increase in mitochondrial respiration, but had no effect on UCP-3 content. Lactate concentration was significantly increased in the culture medium of stimulated cells in the control and Lipovenus groups compared with the respective not-stimulated cells (P < 0.05). We concluded that as mitochondrial function becomes limited by the FFA-uncoupling effect, the ATP demand is mainly supplied by anaerobic glucose metabolism preventing an expected decrease in muscle contraction performance.
机译：检验了以下假设：在电刺激引起的强烈肌肉收缩期间，长链脂肪酸（LCFA）可能会降低线粒体ATP / ADP比率，从而增加糖酵解对ATP产生的贡献。在大鼠中研究了脂质输注（Lipovenus乳剂）对UCP-3 mRNA水平，乳酸，6-磷酸葡萄糖（G-6P）和糖原含量的影响。在休息期间的0、30和60分钟以及在肌肉收缩期间的0、10和20分钟收集用于测定游离脂肪酸和乳酸的血样。还测定了肌肉收缩期后立即收集的比目鱼（SO），红色腓肠肌（RG）和白色腓肠肌（WG）肌肉中乳酸，糖原和G-6P的含量。另外，在肌肉收缩期间确定力水平。还测定了脂质体乳剂对大鼠骨骼肌线粒体呼吸作用以及培养的骨骼肌细胞中UCP-3和乳酸含量的影响。体内实验表明，Lipovenus诱导UCP-3 mRNA水平显着增加。输注Lipovenus后，仅RG肌肉中的乳酸水平增加，而RG和WG肌肉中糖原和G-6P的含量降低（P <0.05）。输注Lipovenus对肌肉力量表现没有任何影响（P> 0.05）。体外实验表明，输注Lipovenus可以明显增加线粒体呼吸，但对UCP-3含量没有影响。与未刺激细胞相比，对照组和Lipovenus组中刺激细胞培养基中的乳酸浓度显着增加（P <0.05）。我们得出的结论是，由于线粒体功能受到FFA解偶联作用的限制，ATP需求主要由厌氧葡萄糖代谢提供，从而阻止了肌肉收缩性能的预期下降。

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