Diverse epigenetic profile of novel human embryonic stem cell lines.

Lagarkova MA; Volchkov PY; Lyakisheva AV; Philonenko ES; Kiselev SL

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Diverse epigenetic profile of novel human embryonic stem cell lines.

机译：新型人类胚胎干细胞系的表观遗传概况。

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Human embryonic stem cells (hESCs) are a promising model for studying mechanisms of regulation of early development and differentiation. OCT4, NANOG, OCT4-related genes and some others were recently described to be important in pluripotency maintenance. Lesser is known about molecular mechanisms involved in their regulation. Apart from genetic regulation of gene expression epigenetic events, particularly methylation, play an important role in early development. Using RT-PCR we studied the expression of pluripotency-related genes OCT4, NANOG, DPPA3 and DPPA5 during hESCs differentiation to embryoid bodies. Analysis of methylation profiles of promoter or putative regulatory regions of the indicated genes demonstrated that expression of the pluripotency-maintaining genes correlated with their methylation status, whereas methylation of DPPA3 and DPPA5 varied between cell lines. We propose that DNA methylation underlies the developmental stage-specific mechanisms of pluripotency-related genes expression and reactivation and may have an impact on differentiation potential of hESC lines.

机译：人类胚胎干细胞（hESCs）是研究早期发育和分化调控机制的有前途的模型。最近描述了OCT4，NANOG，OCT4相关基因以及其他一些基因在多能性维持中很重要。对涉及其调控的分子机制了解较少。除了基因表达的遗传调控外遗传事件，特别是甲基化，在早期发育中起重要作用。使用RT-PCR，我们研究了hESCs向胚状体分化过程中多能性相关基因OCT4，NANOG，DPPA3和DPPA5的表达。分析所示基因的启动子或推定调控区的甲基化图谱表明，维持多能性的基因的表达与其甲基化状态相关，而在细胞系之间，DPPA3和DPPA5的甲基化有所不同。我们建议DNA甲基化奠定了多能性相关基因表达和再激活的发展阶段特定机制的基础，并可能对hESC系的分化潜力产生影响。

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《Cell cycle》 |2006年第4期|共5页
作者
Lagarkova MA; Volchkov PY; Lyakisheva AV; Philonenko ES; Kiselev SL;
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正文语种 eng
中图分类细胞生物学;
关键词
Embryo; Epigenesis; Genetic; Stem Cells; 胚胎; 干细胞;

机译：Embryo;Epigenesis;Genetic;Stem Cells;胚胎;干细胞;

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1. Diverse epigenetic profile of novel human embryonic stem cell lines. [J] . Lagarkova MA, Volchkov PY, Lyakisheva AV, Cell cycle . 2006,第4期

机译：新型人类胚胎干细胞系的表观遗传概况。
2. Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment [J] . Lynsey Howard, Ruth M Mackenzie, Nikolay A Pchelintsev, Stem Cell Research & Therapy . 2013,第2期

机译：人类胚胎干细胞定向内皮分化过程中的转录和表观遗传学变化分析表明FOXA2是早期中胚层承诺的标志
3. Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment [J] . Lynsey Howard, Ruth M Mackenzie, Nikolay A Pchelintsev, Stem Cell Research & Therapy . 2013,第2期

机译：人类胚胎干细胞定向内皮分化过程中的转录和表观遗传学变化分析表明FOXA2是早期中胚层承诺的标志
4. A systems-approach to studying histone H4 and its epigenetic regulatory role in human embryonic stem cells [C] . Justin Brumbaugh, Doug Phanstiel, Samuel S. Oliver, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：一种研究组蛋白H4的系统方法及其在人胚胎干细胞中的表观遗传调控作用
5. Transcriptional and epigenetic dynamics observed during lineage specification of human embryonic stem cells. [D] . Gifford, Casey. 2013

机译：在人类胚胎干细胞谱系指定期间观察到的转录和表观遗传动力学。
6. Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment [O] . Lynsey Howard, Ruth M Mackenzie, Nikolay A Pchelintsev, 2013

机译：人类胚胎干细胞定向内皮分化过程中的转录和表观遗传学变化分析表明FOXA2是早期中胚层承诺的标志
7. Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment [O] . 2013

机译：人类胚胎干细胞定向内皮分化过程中的转录和表观遗传学变化分析表明FOXA2是早期中胚层承诺的标志

Diverse epigenetic profile of novel human embryonic stem cell lines.

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