...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Radiation and Environmental Biophysics >Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926
【24h】

Low-dose irradiation causes rapid alterations to the proteome of the human endothelial cell line EA.hy926

机译：低剂量照射会导致人内皮细胞系EA.hy926的蛋白质组快速改变

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

High doses of ionising radiation damage the heart by an as yet unknown mechanism. A concern for radiological protection is the recent epidemiological data indicating that doses as low as 100-500 mGy may induce cardiac damage. The aim of this study was to identify potential molecular targets and/or mechanisms involved in the pathogenesis of low-dose radiation-induced cardiovascular disease. The vascular endothelium plays a pivotal role in the regulation of cardiac function and is therefore a potential target tissue. We report here that low-dose radiation induced rapid and time-dependent changes in thecytoplasmic proteome of the human endothelial cell line EA.hy926. The proteomes were investigated at 4 and 24 h after irradiation at two different dose rates (Co-60 gamma ray total dose 200 mGy; 20 mGy/min and 190 mGy/min) using 2D-DIGE technology. Differentially expressed proteins were identified, after in-gel trypsin digestion, by MALDI-TOF/TOF tandem mass spectrometry, and peptide mass fingerprint analyses. We identified 15 significantly differentially expressed proteins, of which 10 were up-regulated and 5 down-regulated, with more than ± 1.5-fold difference compared with unexposed cells. Pathways influenced by the low-dose exposures included the Ran and RhoA pathways, fatty acid metabolism and stress response.
机译：高剂量的电离辐射通过一种未知的机制损害了心脏。放射防护的关注点是最近的流行病学数据，表明低至100-500 mGy的剂量可能引起心脏损害。这项研究的目的是确定低剂量辐射诱发的心血管疾病的发病机理中潜在的分子靶标和/或机制。血管内皮在心脏功能的调节中起着关键作用，因此是潜在的靶组织。我们在这里报告，低剂量辐射诱导人内皮细胞系EA.hy926的细胞质蛋白质组的快速和时间依赖性变化。使用2D-DIGE技术以两种不同的剂量率（Co-60γ射线总剂量200 mGy; 20 mGy / min和190 mGy / min）在照射后4和24 h研究蛋白质组。凝胶内胰蛋白酶消化后，通过MALDI-TOF / TOF串联质谱法和肽质量指纹分析鉴定出差异表达的蛋白质。我们鉴定了15个差异显着表达的蛋白质，其中10个上调而5个下调，与未暴露的细胞相比，差异超过±1.5倍。低剂量暴露影响的途径包括Ran和RhoA途径，脂肪酸代谢和应激反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号