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首页> 外文期刊>Cellular Physiology and Biochemistry >Secretin increases the paracellular permeability of CAPAN-1 pancreatic duct cells
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Secretin increases the paracellular permeability of CAPAN-1 pancreatic duct cells

机译：促胰液素增加CAPAN-1胰管细胞的细胞旁通透性

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The effects of secretin, the physiological secretagogue for pancreatic ducts, were studied in CAPAN-1 pancreatic duct carcinoma cells. When grown to confluence on plastic dishes, CAPAN-1 cells form domes and exhibit marked increases in culture content of Na+ and urea distribution space (UDS). This parameter is measured as an index of both intracellular and dome compartments under the conditions adopted. Both Na+ increase and dome formation are inhibited by long term incubation with phorbols, DIDS, DPC, EIPA, H2DIDS, and brefeldin. Short term treatment with secretin or 8-Br-cAMP/teophylline causes dome collapse and a marked decrease in UDS and culture content of Na+. Secretin-induced sodium decrease is not abolished by ion channel inhibitors, suggesting that diffusion routes other than ion channels are involved in hormone effects. This hypothesis is also in agreement with data obtained on CAPAN-1 cells cultured on permeable inserts, where no change in Na+ content or UDS is detected upon secretin treatment. Confluent monolayers exhibit a high transepithelial resistance (R-ms) which is markedly and reversibly decreased by secretin. The hormone also decreases the transepithelial voltage (V-ms) and raises the monolayer permeability to mannitol. It is concluded that secretin enhances the paracellular permeability of pancreatic duct cells. This effect of secretin, unknown thus far, may be involved in the mechanism of pancreatic secretion in vivo. Copyright (C) 2000 S. Karger AG, Basel. [References: 48]
机译：在CAPAN-1胰管癌细胞中研究了促胰液素（胰管的生理促分泌剂）的作用。当在塑料皿上生长到汇合时，CAPAN-1细胞形成圆顶，并显示Na +和尿素分布空间（UDS）的培养物含量显着增加。在所采用的条件下，该参数被测量为细胞内室和圆顶室的指数。通过与佛波醇，DIDS，DPC，EIPA，H2DIDS和布雷菲丁的长期孵育，可以抑制Na +的增加和圆顶的形成。用促胰液素或8-Br-cAMP /茶碱短期治疗会导致穹顶塌陷，UDS和Na +培养物含量显着下降。离子通道抑制剂不能消除促胰液素诱导的钠减少，这表明除离子通道以外的扩散途径也参与激素作用。该假设也与在可渗透插入物上培养的CAPAN-1细胞上获得的数据相符，其中在促胰液素处理后未检测到Na +含量或UDS的变化。汇合的单层细胞表现出较高的跨上皮抵抗力（R-ms），而分泌素显着且可逆地降低了跨上皮抵抗力。该激素还降低了跨上皮电压（V-ms），并提高了对甘露醇的单层渗透性。结论是促胰液素增强胰腺导管细胞的细胞旁通透性。迄今为止尚不清楚的促胰液素的这种作用可能与体内胰腺分泌的机制有关。版权所有（C）2000 S.Karger AG，巴塞尔。 [参考：48]

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