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首页> 外文期刊>Cellular Physiology and Biochemistry >Tyrosine kinases and phosphatases in the estivating spadefoot toad
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Tyrosine kinases and phosphatases in the estivating spadefoot toad

机译：诱人的赤脚蟾蜍中的酪氨酸激酶和磷酸酶

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To endure seasonally arid conditions, spadefoot toads (Scaphiopus couchii) spend 9-10 months underground each year in a hypometabolic state, termed estivation, Protein tyrosine kinases (PTKs) and phosphatases (PTPs) were evaluated in organs of control and estivating toads to assess their possible role in signal transduction during estivation, Total PTK activity decreased by 27-52 % in liver, lung and skeletal muscle during estivation but rose by 66% in heart, Total PTP activity changed only in liver (55 % decrease) and heart (74 % increase), Analysis of the distribution of PTKs between cytoplasmic and membrane-associated forms showed that estivation-link,ed changes in both fractions occurred in heart (increase) and liver (decrease) whereas in lung and skeletal muscle only the soluble fraction was affected, PTPs were assessed using both a general substrate (ENDpYINASL) and a substrate (DADEpYLIPQQG) specific for PTPs containing the SH2 binding site; both revealed estivation-associated changes in activities and subcellular distribution of PTPs in all tissues, C)EAE-Sephadex chromatography showed multiple forms of skeletal muscle PTKs and PTPs in both soluble and insoluble fractions, Each fraction showed three major peaks of PTK activity, two of which shifted in elution position during estivation, The data show that PTKs and PTPs are modified in an organ-specific fashion during estivation by three mechanisms: changes in total activity, changes in subcellular distribution and possible protein covalent modification, Copyright (C) 2001 S. Karger AG, Basel. [References: 34]
机译：为了忍受季节性干旱条件，赤脚蟾蜍（Scaphiopus benchii）每年在地下9到10个月处于代谢不足状态，称其为“陶醉”，并在控制和陶醉蟾蜍的器官中评估了蛋白质酪氨酸激酶（PTK）和磷酸酶（PTP）的含量。它们可能在信号传导过程中发挥作用，在信号传导过程中，肝脏，肺和骨骼肌中的总PTK活性下降了27-52％，但是在心脏中上升了66％，仅在肝脏和心脏中总PTP活性发生了变化（下降了55％）增加74％），对PTK在细胞质和膜相关形式之间分布的分析表明，在心脏（增加）和肝脏（减少）中两个部分都发生了修炼链接的变化，而在肺和骨骼肌中只有可溶性部分感染后，使用通用底物（ENDpYINASL）和特异于含有SH2结合位点的PTP底物（DADEpYLIPQQG）评估PTP;两者都揭示了所有组织中PTP活性和亚细胞分布的着迷相关变化，C）EAE-Sephadex色谱显示可溶和不可溶级分中骨骼肌PTK和PTP的形式多种多样，每个级分均显示PTK活性的三个主要峰，两个数据显示，在耕作过程中，PTK和PTP通过器官特异性方式被三种机制修饰：总活性变化，亚细胞分布变化和可能的蛋白质共价修饰，版权（C）2001 S.Karger AG，巴塞尔。 [参考：34]

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