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Calmodulin-mediated regulation of bicarbonate and lactate transports in rat jejunum

机译：钙调蛋白介导的大鼠空肠中碳酸氢根和乳酸转运的调节

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Background/Aims: Ca2+/CaM is known to modulate the activity of several transport systems and its regulation can be accomplished either directly or via the involvement of specific protein kinases. Aim of this study was to investigate the possible role of Ca2+/CaM on bicarbonate and lactate transports in rat jejunal enterocyte. Methods: Enzymatic assays in isolated plasma membranes were performed. Moreover membrane vesicles, transiently opened and resealed, were loaded with Ca2+ and calmodulin, both in the absence and in the presence of ATP, and were used after that to perform uptake studies Results: Enzymatic assays gave evidence for the presence of Ca2+/CaM-dependent protein kinase II (CaMKII) in plasma membranes from rat jejunum. However, uptake experiments suggest that Ca2+/CaM, and not CaMKII, inhibits both basolateral Cl-/HCO3- exchange and H+-lactate symport, whilst HCO3- and Cl- conductances are unaffected. Neither Ca2+/CaM nor CaMKII seem to regulate brush border Na+/H+ exchanger activity. Conclusion: These data are consistent with a Ca2+/CaM- mediated reduction of bicarbonate and lactate exit from jejunal enterocyte. Copyright (C) 2002 S. Karger AG, Basel. [References: 31]
机译：背景/目的：已知Ca2 + / CaM可调节几种转运系统的活性，其调节可直接或通过特定蛋白激酶的参与来完成。这项研究的目的是调查Ca2 + / CaM对大鼠空肠肠上皮细胞中碳酸氢根和乳酸转运的可能作用。方法：在分离的质膜中进行酶测定。此外，在不存在和存在ATP的情况下，暂时打开并重新密封的膜囊泡均装有Ca2 +和钙调蛋白，之后用于进行摄取研究。结果：酶法分析证明存在Ca2 + / CaM-大鼠空肠质膜中的依赖蛋白激酶II（CaMKII）。但是，摄取实验表明，Ca2 + / CaM而不是CaMKII抑制基底外侧的Cl- / HCO3-交换和H +-乳酸盐共生，而HCO3-和Cl-的电导率不受影响。 Ca2 + / CaM或CaMKII似乎都不能调节刷状边界Na + / H +交换子的活性。结论：这些数据与Ca2 + / CaM介导的空肠肠上皮细胞碳酸氢盐和乳酸出口减少的结果一致。版权所有（C）2002 S.Karger AG，巴塞尔。 [参考：31]

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