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首页> 外文期刊>Cellular Physiology and Biochemistry >Involvement of phosphatidic acid in both degranulation and oxidative activity in fMet-Leu-Phe stimulated polymorphonuclear leukocytes
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Involvement of phosphatidic acid in both degranulation and oxidative activity in fMet-Leu-Phe stimulated polymorphonuclear leukocytes

机译：磷脂酸参与fMet-Leu-Phe刺激的多形核白细胞的脱粒和氧化活性

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Background/Aim: Polymorphonuclear leukocytes (neutrophils) release a variety of toxic agents - proteins and reactive oxygen species (ROS) - that are used to inactivate foreign microorganisms in the non-specific immune response. This study was undertaken to compare intracellular signalling pathways that lead to the ROS production as well as degranulation of azurophilic granules of human fMet-Leu-Phe/cytochalasin B stimulated neutrophils. Methods: Luminol-amplified chemiluminescence was used for monitoring the oxidative activity of human neutrophils in the presence of various inhibitors. The elastase activity was assessed in the neutrophil supernatant as a marker for degranulation of azurophilic granules. Results: Tested inhibitors of enzymes of signalling cascades showed the same effect on the ROS production and on the activity of elastase released from neutrophils. The only difference was obtained with staurosporine: it inhibited the chemiluminescence response, but increased the elastase release. Conclusion: Early signalling pathways leading to the ROS production and the degranulation are ubiquitous in human neutrophils. They are branching most probably at the point of the phosphatidic acid production by phospholipase D. A protein kinase activated by this lipid second messenger might play a central regulatory role in human neutrophils. Copyright (C) 2003 S. Karger AG, Basel. [References: 41]
机译：背景/目的：多形核白细胞（嗜中性粒细胞）释放多种有毒物质-蛋白质和活性氧（ROS）-用于使非特异性免疫反应中的外来微生物失活。进行这项研究以比较导致人fMet-Leu-Phe /细胞松弛素B刺激的嗜中性粒细胞的ROS产生以及嗜酸性颗粒脱粒的细胞内信号通路。方法：在各种抑制剂存在下，使用鲁米诺扩增的化学发光法监测人中性粒细胞的氧化活性。在嗜中性粒细胞上清液中评估弹性蛋白酶活性，作为嗜氮颗粒脱粒的标记。结果：测试的信号传导级联酶抑制剂对ROS的产生和嗜中性粒细胞释放的弹性蛋白酶的活性具有相同的作用。星形孢菌素获得的唯一区别是：它抑制了化学发光反应，但增加了弹性蛋白酶的释放。结论：导致人类中性粒细胞普遍存在导致ROS产生和脱粒的早期信号通路。它们最有可能在磷脂酶D产生磷脂酸的位置分支。由该脂质第二信使激活的蛋白激酶可能在人类嗜中性粒细胞中发挥重要的调节作用。版权所有（C）2003 S.Karger AG，巴塞尔。 [参考：41]

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