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首页> 外文期刊>Cellular Physiology and Biochemistry >Identification of Plateled-derived Growth Factor-BB as Cardiogenesis-Inducing Factor in Mouse Embryonic stem cells under Serum-Free Conditions
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Identification of Plateled-derived Growth Factor-BB as Cardiogenesis-Inducing Factor in Mouse Embryonic stem cells under Serum-Free Conditions

机译：无血清条件下小鼠胚胎干细胞中血小板源性生长因子-BB作为心源性诱导因子的鉴定

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Background/Aims : Embryonic stem (ES) cells may represent an alternative source of functionally mature cardiomyocytes for the treatment of heart diseases. ES cells spontaneously differentiate into spheroidal aggregates, also referred to as embryoid bodies (EBs). The identification of growth factors playing a decisive role in cardiogenesis is a crucial issue for the generation of mature cardiomyocytes. Methods: In order to identify growth factors promoting cardiac development, we established a new differentiation protocol using a defined serum-replacement medium(SRM)containing 5 mug/ml insulin and 5 mug/ml transferrin in combination with Dulbecco's Modified Eagle Medium (DMEM). Furthermore, we added platelet-derived growth factor-BB (PDGF-BB) or sphingosine-1-phosphate(SPP)to promote cardiac differentiation. Results: Using SRM/DMEM, we obtained a 6-fold increase of cardiac specific myosin heavy chain and beta (cMHCalpha/beta) in relation to 0,2% foetal calf serum (FCS) /DMEM ( = 100%). Stimulation of EBs with PDGF-BB in the presence of SRM/DMEM resulted in a further 2,6-fold enhancement in comparison with the SRM/DMEM-induced increase of cMHCalpha/beta (=100%). A parallel increase in the number of beating EBs was observed. Similar results were obtained after stimulation of EBs with 5 mug/ml SPP. Conclusion:We established a serum-free protocol and identify PDGF-BB and SPP as potent factors promoting cardiogenesis in ES cells. Copyright (C) 2003 S. Karger AG, Basel. [References: 26]
机译：背景/目的：胚胎干（ES）细胞可能代表功能性成熟心肌细胞的替代来源，用于治疗心脏病。 ES细胞自发分化为球状聚集体，也称为胚状体（EBs）。鉴定在心肌发生中起决定性作用的生长因子是成熟心肌细胞生成的关键问题。方法：为了确定促进心脏发育的生长因子，我们使用含5杯/毫升胰岛素和5杯/毫升转铁蛋白的明确的血清替代培养基（SRM）结合Dulbecco的改良Eagle培养基（DMEM）建立了新的分化方案。此外，我们添加了血小板衍生的生长因子-BB（PDGF-BB）或鞘氨醇-1-磷酸（SPP）来促进心脏分化。结果：使用SRM / DMEM，相对于0.2％胎牛血清（FCS）/ DMEM（= 100％），我们获得了心脏特异性肌球蛋白重链和β（cMHCalpha / beta）增加了6倍的结果。与SRM / DMEM诱导的cMHCalpha / beta升高（= 100％）相比，在SRM / DMEM存在的情况下用PDGF-BB刺激EB可以进一步提高2,6-倍。观察到跳动的EB数量平行增加。用5杯/毫升SPP刺激EB后获得相似的结果。结论：我们建立了无血清方案，并确定PDGF-BB和SPP是促进ES细胞心脏发生的有效因子。版权所有（C）2003 S.Karger AG，巴塞尔。 [参考：26]

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