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首页> 外文期刊>Cellular Physiology and Biochemistry >Expression of TMEM16A and SLC4A4 in human pancreatic islets
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Expression of TMEM16A and SLC4A4 in human pancreatic islets

机译：TMEM16A和SLC4A4在人胰岛中的表达

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摘要

Stimulation of insulin release by D-glucose is accompanied by Cl ~- and HCO _3 ~- efflux from pancreatic islet cells. The efflux of these anions may involve volume-regulated anion channels, including possibly TMEM16A, and the Na ~+-HCO _3 ~--cotransporter SLC4A4. The present study was designed to explore the expression of both TMEM16A and SLC4A4 in human pancreatic islets. Methods: Pancreases were obtained from human cadaveric donors. Immunodetection of TMEM16A and SLC4A4 was performed by immunohistochemistry on sections of fixed pancreas, while real-time PCR for the study of corresponding gene expression was performed on RNA extracted from both total pancreatic pieces and isolated pancreatic islets. Results: RT-PCR yielded lower levels of SLC4A4 in isolated islets than in the total pancreas, whilst a mirror image prevailed for TMEM16A mRNA. Immunohistochemistry of human pancreas, however, indicated comparable immunostaining of SLC4A4 in insulin-producing cells and exocrine pancreatic cells, whilst that of TMEM16A appeared less pronounced in insulin-producing cells than in exocrine cells. Conclusion: The present findings support the view that, in humans like in rodent, the regulation of anion fluxes in insulin-producing cells may involve both SLC4A4 and TMEM16A.
机译：D-葡萄糖刺激胰岛素释放伴随着胰岛细胞Cl〜-和HCO_3〜-流出。这些阴离子的流出可能涉及体积调节的阴离子通道，包括可能的TMEM16A和Na〜+ -HCO_3〜-共转运蛋白SLC4A4。本研究旨在探讨TMEM16A和SLC4A4在人胰岛中的表达。方法：从人体尸体供体中获得胰腺。 TMEM16A和SLC4A4的免疫检测是通过免疫组织化学法在固定的胰腺切片上进行的，而实时PCR技术则是对从总胰腺碎片和分离的胰岛中提取的RNA进行相应基因表达的研究。结果：RT-PCR在分离的胰岛中产生的SLC4A4水平低于总胰岛中的水平，而TMEM16A mRNA普遍存在镜像。然而，人胰腺的免疫组织化学表明，在产生胰岛素的细胞和外分泌胰腺细胞中，SLC4A4的免疫染色相当，而在产生胰岛素的细胞中，TMEM16A的免疫染色不如在外分泌细胞中明显。结论：本研究结果支持这样的观点，即在人类中，例如在啮齿动物中，胰岛素产生细胞中阴离子通量的调节可能涉及SLC4A4和TMEM16A。

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