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首页> 外文期刊>Cellular Physiology and Biochemistry >MiR-125b Inhibits Tumor Growth and Promotes Apoptosis of Cervical Cancer Cells by Targeting Phosphoinositide 3-Kinase Catalytic Subunit Delta
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MiR-125b Inhibits Tumor Growth and Promotes Apoptosis of Cervical Cancer Cells by Targeting Phosphoinositide 3-Kinase Catalytic Subunit Delta

机译：MiR-125b通过靶向磷酸肌醇3-激酶催化亚基Delta抑制肿瘤生长并促进宫颈癌细胞凋亡。

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摘要

Backgroud: microRNAs (miRNAs) are involved in cancer-related processes. The miRNA-125b (miR-125b) has been identified as miRNA over-expressed in a wide variety of cancers. However, the role of miR-125b in the context of cervical carcinoma remains unknown. Methods: In this study, the effect of miR-125b on the proliferation and apoptosis of human cervical cells was analyzed by MTT assay and Flow cytometry analysis. we identified phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) as a novel miR-125b target. Results: overexpression of miR-125b in HeLa cervical cancer cells decreased cell proliferation, induced apoptosis and down-regulated expression of PIK3CD. To identify the mechanisms responsible, we investigated the PI3K/Akt pathway and found that PI3K, phospho-Akt, and phospho-mTOR were all down-regulated, while Bid was up-regulated in miR-125b-overexpressing subclones. In vivo, over expression of miR-125b in HeLa cells markedly reduced their ability to form tumors. Conclusion: these results suggest that miR-125b suppresses tumor growth activity by targeting the PI3K/Akt/mTOR signal-ing pathway, and may provide a target for effective therapies.
机译：背景：microRNA（miRNA）参与了与癌症相关的过程。 miRNA-125b（miR-125b）已被鉴定为在多种癌症中过表达的miRNA。但是，miR-125b在宫颈癌中的作用仍然未知。方法：采用MTT法和流式细胞仪分析miR-125b对人宫颈细胞增殖和凋亡的影响。我们确定了磷酸肌醇3-激酶催化亚基三角洲（PIK3CD）作为新型miR-125b目标。结果：HeLa宫颈癌细胞中miR-125b的过表达降低了细胞增殖，诱导了细胞凋亡并下调了PIK3CD的表达。为了确定负责的机制，我们调查了PI3K / Akt途径，发现PI3K，磷酸化Akt和磷酸化mTOR均被下调，而Bid在miR-125b过表达的亚克隆中被上调。在体内，miR-125b在HeLa细胞中的过度表达明显降低了它们形成肿瘤的能力。结论：这些结果表明，miR-125b通过靶向PI3K / Akt / mTOR信号传导途径抑制肿瘤生长活性，并可能为有效疗法提供靶点。

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