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首页> 外文期刊>Biological trace element research >Microcalorimetric study of the action of Ce(III) ions on the growth of E. coli.
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Microcalorimetric study of the action of Ce(III) ions on the growth of E. coli.

机译：微量热法研究Ce（III）离子对大肠杆菌生长的作用。

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A microcalorimetric technique based on bacterial heat output was used to evaluate the action of Ce(III) ions on the growth of Escherichia coli. The power-time curves of the growth metabolism of the bacteria were studied in the presence and absence of Ce(III) by means of a LKB-2277 Bioactivity Monitor, by a stopped-flow method at 37 degrees C. For evaluation of the results, the maximum power, Pmax, the growth rate constant k, and the heat effects Qlog, Qstat, and Qtot for the log phase, the stationary phase, and total heat output, respectively, were determined. For comparison, a spectrophotometer was used to estimate the number of cells in the liquid culture. The shape of the bacteria was examined by electron microscopy. We concluded that the presence of cerium ions at concentrations below 350 microg/mL have a stimulatory effect on the growth of E. coli, whereas concentrations at or above 400 microg/mL may have an inhibitory effect.
机译：基于细菌热量输出的微量量热技术用于评估Ce（III）离子对大肠杆菌生长的作用。在37℃下，通过LKB-2277生物活性监测仪，通过停流法，研究了在有或没有铈（III）存在下，细菌生长代谢的功率-时间曲线。 ，分别确定了对数相，固定相和总热量输出的最大功率Pmax，增长率常数k以及热效应Qlog，Qstat和Qtot。为了进行比较，使用分光光度计来估计液体培养物中的细胞数量。通过电子显微镜检查细菌的形状。我们得出的结论是，浓度低于350微克/毫升的铈离子的存在对大肠杆菌的生长具有刺激作用，而浓度大于或等于400微克/毫升的铈离子可能具有抑制作用。

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