• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Respirology : >Denopamine stimulates alveolar fluid clearance via cystic fibrosis transmembrane conductance regulator in rat lungs.
【24h】

Denopamine stimulates alveolar fluid clearance via cystic fibrosis transmembrane conductance regulator in rat lungs.

机译：地诺明通过大鼠肺中的囊性纤维化跨膜电导调节剂刺激肺泡液清除。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

OBJECTIVE: The objective of this study was to test the hypothesis that cystic fibrosis transmembrane conductance regulator (CFTR) plays a role in beta(1)-adrenergic agonist-stimulated alveolar fluid clearance. METHODS: Isotonic 5% albumin solutions containing different pharmacological agents were instilled into the alveolar spaces of the isolated rat lungs. The lungs were inflated with 100% oxygen at an airway pressure of 7 cm H(2)O and placed in a humidified incubator at 37 degrees C. Alveolar fluid clearance was estimated by the progressive increase in the albumin concentration over 1 h. To test the hypothesis, we determined whether CFTR Cl(-) channel inhibitors (glibenclamide and CFTR(inh)-172) inhibited the effect of denopamine, a beta(1)-adrenergic agonist, on stimulation of alveolar fluid clearance in the isolated rat lungs. RESULTS: Denopamine increased alveolar fluid clearance in a dose-dependent manner. Atenolol, a beta(1)-adrenergic antagonist, abolished the effects of denopamine on stimulation of alveolar fluid clearance. Although glibenclamide alone or CFTR(inh)-172 alone did not change basal alveolar fluid clearance, these CFTR inhibitors inhibited the effect of denopamine on alveolar fluid clearance. CONCLUSION: CFTR plays a role in beta(1)-adrenergic agonist-stimulated alveolar fluid clearance in rat lungs.
机译：目的：本研究的目的是检验以下假设：囊性纤维化跨膜电导调节剂（CFTR）在β（1）-肾上腺素能激动剂刺激的肺泡液清除中起作用。方法：将含有不同药理剂的等渗5％白蛋白溶液滴入离体大鼠肺的肺泡腔中。在7 cm H（2）O的气道压力下向肺中充入100％的氧气，然后将其置于37摄氏度的加湿培养箱中。通过1小时内白蛋白浓度的逐渐升高来估计肺泡液清除率。为了验证该假设，我们确定了CFTR Cl（-）通道抑制剂（glibenclamide和CFTR（inh）-172）是否抑制了β（1）-肾上腺素能激动剂denopamine刺激离体大鼠肺泡液清除的作用肺。结果：地那巴胺以剂量依赖性方式增加肺泡液清除率。阿替洛尔，一种β（1）-肾上腺素拮抗剂，废除了多巴酚丁胺对刺激肺泡液清除的影响。尽管单独使用格列本脲或单独使用CFTR（inh）-172不会改变基底牙槽腔清除率，但这些CFTR抑制剂抑制了denopamine对肺泡液清除率的影响。结论：CFTR在大鼠肺中β（1）-肾上腺素能激动剂刺激的肺泡液清除中起作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号