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首页> 外文期刊>Cell cycle >Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhimurium.
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Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhimurium.

机译：全身性靶向鼠伤寒沙门氏菌的裸鼠的原发性骨肿瘤和高级别骨肉瘤的肺转移。

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摘要

We report here a new targeting strategy for primary bone tumor and lung metastasis with a modified auxotrophic strain of Salmonella typhimurium. We have previously developed the genetically-modified strain of S. typhimurium, selected for tumor targeting and therapy in vivo. Normal tissue is cleared of these bacteria even in immunodeficient athymic mice with no apparent side effects. In this study, the tumor-targeting strain of S. typhimurium, termed A1-R, was administered i.v. to nude mice which have primary bone tumor and lung metastasis. Primary bone tumor was obtained by orthotopic intra-tibial injection of 5 x 10(5) 143B-RFP (red fluorescent protein) human osteosarcoma cells. One group of mice was treated with A1-R expressing GFP (green fluorescent protein) and another group was used a as control. A1-R (5 x 10(7) colony-forming units) was injected in the tail vein three times on a weekly basis. On day 28, lung samples were excised and observed with the Olympus OV100 Small Animal Imaging System. Thesize of the primary tumor and RFP intensity of lung metastasis were measured. Primary bone tumor size (fluorescence area [mm(2)]) was 232 +/- 70 in the untreated group and 95 +/- 23 in the treated group (p < 0.05). RFP intensity of the lung metastasis was 3 +/- 1.5 x 10(6) in the untreated group and 0.42 +/- 0.33 x 10(6) in the treated group (p < 0.05). Therefore, bacterial treatment was effective for both primary bone tumor and lung metastasis.
机译：我们在这里报告了一种新的靶向策略，用于改良鼠伤寒沙门氏菌的营养缺陷型原发性骨肿瘤和肺转移。我们以前已经开发了鼠伤寒沙门氏菌的基因修饰菌株，选择用于肿瘤靶向和体内治疗。即使在免疫缺陷的无胸腺小鼠中，正常组织也清除了这些细菌，而没有明显的副作用。在这项研究中，鼠伤寒沙门氏菌的肿瘤靶向菌株被称为A1-R。具有原发性骨肿瘤和肺转移的裸鼠。原位骨肿瘤是通过原位胫骨内注射5 x 10（5）143B-RFP（红色荧光蛋白）人骨肉瘤细胞获得的。一组小鼠用表达A1-R的GFP（绿色荧光蛋白）处理，另一组用作对照组。每周向尾静脉注射A1-R（5 x 10（7）个菌落形成单位）。在第28天，切除肺样品并用奥林巴斯OV100小型动物成像系统观察。测量原发肿瘤的大小和肺转移的RFP强度。未治疗组的原发性骨肿瘤大小（荧光面积[mm（2）]）为232 +/- 70，治疗组为95 +/- 23（p <0.05）。未治疗组的肺转移RFP强度为3 +/- 1.5 x 10（6），治疗组为0.42 +/- 0.33 x 10（6）（p <0.05）。因此，细菌治疗对于原发性骨肿瘤和肺转移都是有效的。

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