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首页> 外文期刊>Resuscitation. >Evaluation of a tape removal test to assess neurological deficit after cardiac arrest in rats.
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Evaluation of a tape removal test to assess neurological deficit after cardiac arrest in rats.

机译：评估大鼠心脏骤停后的胶带去除试验以评估神经功能缺损。

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BACKGROUND: In animal models of cardiocirculatory arrest (CA) it is of major interest to establish tests that can assess neurological damage after global cerebral ischaemia following CA. We evaluated a tape removal test with regard to detection of sensorimotor deficit, comparing it to the Neurological Deficit Score (NDS) in an established model of global cerebral ischaemia after CA in rats. METHODS: Rats were subjected to either 6 min of CA followed by cardiopulmonary resuscitation (CPR) or a sham operation. At 1, 3 and 7 days from the intervention, two different neurological tests were applied to all animals: in the tape removal test, the time was measured from attachment of adhesive tapes to the front paws until the animals removed them using their teeth and compared to latencies in the sham group. The NDS assessed two parameters ("travel beam" and "stop at the edge of a table"). Receiver operating characteristic (ROC) analysis was used to compare tests. RESULTS: In the tape removal test, all animalsof the CPR group showed a clear neurological deficit throughout the observation period with a marked recovery until day 7 (pre-CA: 4s, 1 day: 180 s, 3 days: 165 s, 7 days: 44 s; data are median values). Latencies differed significantly from those of sham-operated animals (1 day: P<0.001, 3 days: P=0.003, 7 days: P=0.006). ROC analysis showed that the tape removal test but not the NDS was appropriate for detecting neurological damage 3 and 7 days after restoration of spontaneous circulation (ROSC). Histological examination confirmed neuronal damage to the hippocampus, cortex, thalamus and striatum. CONCLUSION: In the present study, a clinically relevant sensorimotor deficit after global cerebral ischaemia following cardiac arrest in rats has been quantified for the first time by using a tape removal test. The tape removal test is a sensitive method that can be easily applied to test large numbers of animals in future studies.
机译：背景：在心脏循环骤停（CA）的动物模型中，建立可评估CA继发性全脑缺血后神经系统损害的测试尤为重要。我们评估了关于感觉运动功能缺陷检测的胶带去除试验，并将其与大鼠CA后全脑缺血建立模型中的神经功能缺损评分（NDS）进行了比较。方法：大鼠接受6分钟的CA，然后进行心肺复苏（CPR）或假手术。在干预的第1、3和7天，对所有动物进行了两种不同的神经系统测试：在胶带去除测试中，测量从将胶带粘贴到前爪直到动物用牙齿将它们去除之间的时间。假组的等待时间。 NDS评估了两个参数（“行进光束”和“停在桌子边缘”）。接收器工作特性（ROC）分析用于比较测试。结果：在胶带剥离试验中，CPR组的所有动物在整个观察期内均表现出明显的神经功能缺损，直至第7天均有明显恢复（CA前：4s，1天：180 s，3天：165 s，7天） ：44 s;数据为中值）。潜伏期与假手术动物的潜伏期显着不同（1天：P <0.001，3天：P = 0.003，7天：P = 0.006）。 ROC分析表明，自发循环（ROSC）恢复后第3天和第7天，胶带剥离试验而非NDS适于检测神经系统损伤。组织学检查证实对海马，皮层，丘脑和纹状体的神经元有损害。结论：在本研究中，首次通过胶带剥离试验量化了大鼠心脏骤停后全脑缺血后临床相关的感觉运动缺陷。胶带去除测试是一种敏感的方法，可以在将来的研究中轻松应用于测试大量动物。

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