...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Review in aquaculture >Proliferating cell nuclear antigen and Vasa protein expression during gonadal development and sexual differentiation in cultured Siberian (Acipenser baerii Brandt, 1869) and Russian (Acipenser gueldenstaedtii Brandt & Ratzeburg, 1833) sturgeon
【24h】

Proliferating cell nuclear antigen and Vasa protein expression during gonadal development and sexual differentiation in cultured Siberian (Acipenser baerii Brandt, 1869) and Russian (Acipenser gueldenstaedtii Brandt & Ratzeburg, 1833) sturgeon

机译：在养殖的西伯利亚（（Acipenser baerii Brandt，1869）和俄罗斯（（Acipenser gueldenstaedtii Brandt＆Ratzeburg，1833）的urge鱼的性腺发育和性分化过程中增殖细胞核抗原和Vasa蛋白的表达

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Early gonadal development in Russian (Acipenser gueldenstaedtii Brandt, 1869) and Siberian sturgeon (Acipenser baerii Brandt & Ratzeburg, 1833) reared in controlled conditions from dph 1-439 (days post hatching) was studied using histological methods. Migrating primordial germ cells (PGCs) were observed in both species on dph 1. On dph 25 in both species gonad primordia developed in gonadal ridges. In dph 115 juveniles two types of gonad were distinguished, putative ovary and putative testicle. Meiotic activity was observed in ovaries on dph 197, while in testicles the first meiotic divisions were observed much later, on dph 439 and only in several Siberian specimens. The presence of Vasa protein was confirmed exclusively in germ cells during gonadogenesis, indicating that Vasa is a good marker of germinal cells also in Acipenseridae. Vasa protein displayed a nuage association or nuage-like localization. Some differences in the level of Vasa protein expression during male and female gonad development were also observed. In the gonads of both investigated sturgeon species the PCNA (proliferating cell nuclear antigen)-positive reaction was observed first in oogonia, while in spermatogonia at approximately 115 dph which was correlated with active mitotic divisions of germ cells. Surprisingly, meiotic oocytes arrested in diplotene also demonstrated strong PCNA-positive germinal vesicle.
机译：使用组织学方法研究了在受控条件下从dph 1-439（孵化后的天数）饲养的俄语（Acipenser gueldenstaedtii Brandt，1869年）和西伯利亚st（Acipenser baerii Brandt＆Ratzeburg，1833年）的早期性腺发育。在dph 1上，在两个物种中均观察到迁移的原始生殖细胞（PGC）。在两个物种的dph 25中，在性腺脊中发育的性腺原基。在dph 115中，区分了两种类型的性腺，即卵巢和睾丸。在dph 197卵巢中观察到减数分裂活性，而在睾丸中，在dph 439上仅在几个西伯利亚标本中观察到了最早的减数分裂分裂。在性腺发生过程中仅在生殖细胞中证实了Vasa蛋白的存在，这表明Vasa也是Acipenseridae中生殖细胞的良好标记。瓦萨蛋白表现出小数关联或小数样定位。在男性和女性性腺发育过程中，Vasa蛋白表达水平也存在一些差异。在两个被研究的st鱼物种的性腺中，首先在卵小卵菌中观察到PCNA（增殖细胞核抗原）阳性反应，而在精原细胞中大约115 dph观察到这是阳性反应，这与生殖细胞的有丝分裂活跃有关。令人惊讶的是，停在二倍体中的减数分裂卵母细胞也显示出强的PCNA阳性生发囊泡。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号