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首页> 外文期刊>Reviews in Toxicology >Estimation of glucosinolates in rapeseed - a review
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Estimation of glucosinolates in rapeseed - a review

机译：油菜籽中芥子油苷的估计-审查

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Among the antinutritional factors present in rapeseed, glucosinolates are the ones in which scientists are more interested than others due to their toxic and goitrogenic effects. Various methods have been developed from time to time to estimate glucosinolates in rapeseed, ranging from simple test paper, enzymatic, volumetric methods to complex HPLC, ELISA, chromatographic methods. But most of the methods developed so far suffer from one or another drawback. Even today, a need exists to develop a simple, rapid, accurate, economical and reliable method. These methods are mainly classified into two types: i) Estimation of individual glucosinolates - which are time-consuming but give qualitative as well as quantitative measurement of glucosinolates; and ii) Estimation of total glucosinolates - which are rapid and advantageous when large numbers of samples are to be analysed for screening purposes. It gives only quantitative data. Important steps of Analysis of glucosinolates are i) Extraction of glucosinolates; ii) Removal of interfering compounds; and iii) Analysis of glucosinolates. Gas chromatography (GC) and high performance liquid chromatography (HPLC) are the most widely used methods. Lack of pure reference glucosinolates is also one of the major difficulties facing any analyst. In the present context, an attempt has been made to review various methods developed and modified till now for estimation of glucosinolates in rapeseed along with their efficiency and drawbacks. Analytical methodologyfor glucosinolates is continuing to evolve and improve and undoubtedly new methods will appear which will further facilitate analysis.
机译：在油菜籽中存在的抗营养因子中，芥子油苷因其毒性和促甲状腺激素作用而比其他人更受关注。时不时地开发了各种方法来评估油菜籽中的芥子油苷，从简单的试纸，酶法，体积法到复杂的HPLC，ELISA，色谱法。但是迄今为止开发的大多数方法都存在一个或另一个缺点。即使在今天，仍然需要开发一种简单，快速，准确，经济和可靠的方法。这些方法主要分为两种类型：i）估计各个芥子油苷-费时但对芥子油苷进行定性和定量测量; ii）估计总的芥子油苷-当要分析大量样品进行筛选时，这是快速且有利的。它仅提供定量数据。芥子油苷分析的重要步骤是：i）芥子油苷的提取; ii）去除干扰化合物; iii）芥子油苷的分析。气相色谱（GC）和高效液相色谱（HPLC）是使用最广泛的方法。缺乏纯参考芥子油苷也是任何分析人员面临的主要困难之一。在目前的背景下，已经尝试审查迄今为止开发和修改的用于估计油菜籽中的芥子油苷的各种方法及其效率和缺点。芥子油苷的分析方法正在不断发展和完善，毫无疑问将出现新方法，这将进一步促进分析。

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