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首页> 外文期刊>Cellular Physiology and Biochemistry >Transduction for sweet taste of saccharin may involve both inositol 1,4,5-trisphosphate and cAMP pathways in the fungiform taste buds in C57BL mice.
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Transduction for sweet taste of saccharin may involve both inositol 1,4,5-trisphosphate and cAMP pathways in the fungiform taste buds in C57BL mice.

机译：糖精甜味的转导可能涉及C57BL小鼠的真菌状味蕾中的肌醇1,4,5-三磷酸和cAMP途径。

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摘要

The transduction pathways for sweet and bitter tastes were investigated with assays of inositol 1,4,5-trisphosphate (IP3) and cyclic adenosine monophosphate (cAMP) levels in mouse fungiform taste buds. Recordings of taste responses were also made in the chorda tympani nerve. Stimulation of the tongue with saccharin elicited a significant increase in IP3 levels in the fungiform papilla only at 20 mM but in cAMP levels at 3 and 20 mM, without affecting those of the nonsensory epithelial tissue. Formation of both IP3 and cAMP induced by 20 mM saccharin was suppressed by pretreatment of the tongue with pronase, a proteolytic enzyme which specifically inhibits sweet responses. Quinine and denatonium elicited both significant increases in IP3 levels at a concentration of 20 mM and slight decreases in cAMP levels at concentrations of 1-20 mM in the fungiform papilla. Recording of the chorda tympani nerve showed good responses by saccharin, quinine, and denatonium at concentrations of 1 mM and higher. These results suggest that the fungiform taste cells in C57BL mice have pronase-sensitive receptors for saccharin, coupled to both the IP3 and the cAMP pathways; the former participates only at high concentration, while the latter acts from low to high concentrations. The results also do not rule out the possibility that a phosphodiesterase-mediated cAMP decrease may be involved in bitter transduction for quinine and denatonium.
机译：通过测定小鼠真菌形味蕾中肌醇1,4,5-三磷酸（IP3）和环状腺苷单磷酸（cAMP）的含量来研究甜味和苦味的转导途径。还记录了鼓膜鼓膜神经中的味觉反应。用糖精刺激舌头仅在20 mM时引起真菌状乳头中IP3的水平显着增加，而在3和20 mM时引起cAMP的水平显着增加，而不会影响非感觉上皮组织的IP3水平。通过20nM糖精诱导的IP3和cAMP的形成，可以通过用脯氨酸酶（一种专门抑制甜味反应的蛋白水解酶）预处理舌头来抑制。奎宁和地那铵在真菌状乳头中均引起IP3浓度在20 mM时显着增加，而cAMP浓度在1-20 mM时则略有下降。鼓膜鼓膜神经的记录显示糖精，奎宁和地那铵在1 mM或更高浓度下具有良好的响应。这些结果表明，C57BL小鼠中的真菌样味觉细胞具有糖精的脯氨酸酶敏感受体，并与IP3和cAMP途径偶联。前者仅在高浓度下参与，而后者则从低浓度到高浓度参与。结果也并不排除磷酸二酯酶介导的cAMP降低可能与奎宁和地那铵的苦味转导有关。

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