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首页> 外文期刊>Cellular Physiology and Biochemistry >Regulation of sorbitol efflux in different renal medullary cells: Similarities and diversities [Review]
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Regulation of sorbitol efflux in different renal medullary cells: Similarities and diversities [Review]

机译：山梨醇在不同肾髓质细胞中的外流调节：相似性和多样性[综述]

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It is well accepted that organic osmolytes, including sorbitol, play a major role in the volume regulation of renal medullary cells. The signal leading to an activation of release channels during RVD is, however, poorly understood. Hypotonicity induced sorbitol efflux was investigated in freshly isolated rat inner medullary collecting duct (IMCD) cells and in rabbit medullary thick ascending limb of Henle's loop (TALH) cells biochemically or using labeled sorbitol. The time course of release was compared with changes in cell volume, measured by confocal microscopy, and alterations in cell calcium (Ca-i) determined by Fura 2 technology. In IMCD cells sorbitol release, volume decrease and Ca-i transients show a close temporal correlation. In addition increases in Ca-i without volume changes stimulate sorbitol efflux. In TALH cells sorbitol release starts after a significant lag time and reaches a maximum when cell volume is already partially restored. The same discrepancy is observed with regard to changes in Ca-i and sorbitol efflux. These studies suggest that in IMCD cells changes in Ca-i are the main regulator for the sorbitol permeability of the plasma membrane. The sorbitol channel present in TALH cells seems to operate predominantly independently of Ca-i. Despite this diversity in signal transduction the sorbitol channels in both renal cell types appear, however, not to be stretch-activated. Copyright (C) 2000 S. Karger AG Basel. [References: 47]
机译：公认的是，包括山梨糖醇在内的有机渗透物在肾髓样细胞的体积调节中起主要作用。然而，在RVD期间导致释放通道激活的信号知之甚少。用生化方法或使用标记的山梨糖醇对新鲜分离的大鼠内髓收集管（IMCD）细胞和亨利环（TALH）细胞的兔髓厚上肢进行了低渗诱导的山梨醇外排。将释放的时间过程与通过共聚焦显微镜测量的细胞体积变化以及通过Fura 2技术确定的细胞钙（Ca-i）变化进行比较。在IMCD细胞中，山梨糖醇释放，体积减少和Ca-i瞬变显示出紧密的时间相关性。另外，在不改变体积的情况下增加Ca-i会刺激山梨糖醇外排。在TALH细胞中，山梨糖醇的释放在相当长的滞后时间后开始，并在细胞体积已部分恢复时达到最大值。关于Ca-i和山梨糖醇外排的变化，观察到相同的差异。这些研究表明，在IMCD细胞中，Ca-i的改变是质膜的山梨醇渗透性的主要调节剂。 TALH细胞中存在的山梨糖醇通道似乎主要独立于Ca-i运作。尽管信号转导具有这种多样性，但是两种肾细胞类型中的山梨糖醇通道似乎都没有被拉伸激活。版权所有（C）2000 S.Karger AG Basel。 [参考：47]

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