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首页> 外文期刊>Rheumatology >Altered B cell balance, but unaffected B cell capacity to limit monocyte activation in anti-neutrophil cytoplasmic antibody-associated vasculitis in remission
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Altered B cell balance, but unaffected B cell capacity to limit monocyte activation in anti-neutrophil cytoplasmic antibody-associated vasculitis in remission

机译：B细胞平衡改变，但不受B细胞限制抗中性粒细胞胞浆抗体相关血管炎中单核细胞活化的能力的缓解

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Objective: Regulatory B cells (Bregs) constitute a subset of B cells with immunomodulatory properties. Numerical and functional alterations in the Breg compartment have been associated with autoimmunity. The aim of this study was to assess the frequency and function of Bregs in patients with ANCA-associated vasculitis (AAV). Methods: B cell subsets were determined in the peripheral blood of 48 AAV patients (12 active, 36 in remission) and 41 healthy controls (HCs) by flow cytometry. Bregs were defined within the CD19+ population as CD24hiCD38hi or CD24hiCD27+ cells. The percentage of IL-10-positive B cells in circulation was analysed by flow cytometry. Sorted CD19+ B cells were co-cultured with monocytes to evaluate their capacity to inhibit monocyte TNF-α production upon lipopolysaccharide stimulation. Results: The frequency of circulating CD19+CD24hiCD38hi cells was not different in AAV patients in remission compared with HCs, but was decreased in patients with active disease [mean in HCs 5.5% (S.D. 1.6) vs active 3.8% (S.D. 2.8), P = 0.0104]. Furthermore, the percentage of CD19+CD24hiCD27+ cells was significantly decreased in both remission and active patients when compared with HCs [HCs 15.0% (S.D. 9.3) vs remission 6.6% (S.D. 4.4) (P0.0001) vs active 6.4% (S.D. 6.2) (P = 0.0006)]. The frequency of IL-10- positive B cells was comparable between patients and HCs. B cells from AAV patients suppressed monocyte TNF-α production to a similar extent to cells from HCs. Conclusion: Based on immunophenotypic classification, Bregs are numerically diminished in AAV patients. However, B cell function in terms of IL-10 production and their capacity to suppress monocyte activation is not compromised in AAV patients in remission.
机译：目的：调节性B细胞（Bregs）构成具有免疫调节特性的B细胞的一个子集。 Breg区室的数字和功能改变与自身免疫有关。这项研究的目的是评估ANCA相关血管炎（AAV）患者中Breg的频率和功能。方法：通过流式细胞术测定48例AAV患者（12例活跃，36例缓解）和41例健康对照（HCs）外周血中的B细胞亚群。 Bregs在CD19 +群体中定义为CD24hiCD38hi或CD24hiCD27 +细胞。通过流式细胞术分析循环中IL-10阳性B细胞的百分比。将分选的CD19 + B细胞与单核细胞共培养，以评估其在脂多糖刺激下抑制单核细胞TNF-α产生的能力。结果：缓解的AAV患者的循环CD19 + CD24hiCD38hi细胞频率与HCs无差异，但活动性疾病患者的循环CD19 + CD24hiCD38hi细胞频率降低[HCs平均值为5.5％（SD 1.6）vs活动性3.8％（SD 2.8），P = 0.0104]。此外，与HCs相比，缓解和活动患者中CD19 + CD24hiCD27 +细胞的百分比均显着降低[HCs 15.0％（SD 9.3）vs缓解6.6％（SD 4.4）（P <0.0001）vs活动6.4％（SD 6.2） ）（P = 0.0006）]。患者与HCs之间IL-10阳性B细胞的频率相当。 AAV患者的B细胞抑制单核细胞TNF-α产生的程度与HCs细胞类似。结论：根据免疫表型分类，AAV患者的Breg数量减少。然而，在缓解的AAV患者中，在IL-10产生方面的B细胞功能及其抑制单核细胞活化的能力并未受到损害。

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