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Label-free C-reactive protein SERS detection with silver nanoparticle aggregates

机译：银纳米粒子聚集体的无标记C反应蛋白SERS检测

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摘要

In this work, we report a qualitative approach for detecting the adsorption of C-reactive protein on phosphocholine-terminated self-assembled monolayers without the use of any labels. An amplified plasmon of concentration-induced silver nanoparticle aggregates located similar to 4.0 nm away from the C-reactive protein via the phosphocholine-terminated self-assembled monolayer linker is considered to be the source of the robust electromagnetic enhancement. The high level (10(9) to 10(10) M-1) of apparent binding constant (K-A) of C-reactive protein suggests that the immobilized surface was well-oriented without extreme random stacking. A Raman sensitivity toward the C-reactive protein around 2800-3000 cm(-1) was noted, which gradually increased upon the addition of successive layers up to approximately 6-7 layers of phosphocholine-coated silver nanoparticle aggregates, with minimum detection amounts of similar to 0.01 ng mL(-1) in buffer and similar to 0.1 ng mL(-1) in 1% serum. A cross-reactivity test confirmed the excellent selectivity and specificity of the measured signals. A computational study based on the finite-difference-time-domain method successfully demonstrated the enhanced (similar to 1.1 x 10(6)) electromagnetic field of the 2-D silver nanoparticle aggregates as compared with that of isolated particles, and was congruent with the analytical enhancement factor (1.7 x 10(5)).
机译：在这项工作中，我们报告了一种定性方法，无需使用任何标记物即可检测C反应蛋白在磷酸胆碱封端的自组装单分子膜上的吸附。浓度诱导的银纳米粒子聚集体的放大等离子体激元通过磷胆碱封端的自组装单层连接子距离C反应蛋白约4.0 nm，被认为是强大的电磁增强源。 C反应蛋白的表观结合常数（K-A）的高水平（10（9）至10（10）M-1）表明固定化的表面取向良好，没有极端的随机堆积。注意到对C反应蛋白的拉曼敏感性约为2800-3000 cm（-1），随着添加连续层而逐渐增加，直至大约6-7层磷胆碱涂层的银纳米颗粒聚集体，且最小检测量为类似于缓冲液中的0.01 ng mL（-1），类似于1％血清中的0.1 ng mL（-1）。交叉反应测试证实了所测信号的出色选择性和特异性。基于时域有限差分法的计算研究成功地证明了二维银纳米粒子聚集体与孤立粒子相比具有增强的（类似于1.1 x 10（6））电磁场，并且与分析增强因子（1.7 x 10（5））。

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