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首页> 外文期刊>RSC Advances >Effects of osmolytes on protein-solvent interactions in crowded environments: study of sucrose and trehalose effects on different proteins by solvent interaction analysis
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Effects of osmolytes on protein-solvent interactions in crowded environments: study of sucrose and trehalose effects on different proteins by solvent interaction analysis

机译：渗透剂对拥挤环境中蛋白质-溶剂相互作用的影响：通过溶剂相互作用分析研究蔗糖和海藻糖对不同蛋白质的影响

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摘要

Partitioning of 11 different proteins and 30 small organic compounds was examined in aqueous dextran-PEG-sodium/potassium phosphate buffer (0.01 M K/NaPB, pH 7.4) two-phase systems (ATPSs) containing 0.5 M sucrose or 0.5 M trehalose. The data obtained were compared to those reported previously for the same compounds and proteins in osmolyte-free ATPS and ATPS containing 0.5 M TMAO (Breydo et al. (2015) Archives of Biochemistry and Biophysics. in press), and analyzed in terms of the so-called Collander linear solvent regression relationship. It was found that the logarithms of the partition coefficients of proteins in the presence of 0.5 M sucrose and trehalose are linearly interrelated. The structural distances of protein 3D structures relative to that of ribonuclease B were estimated. These estimates were shown to be linearly related to the previously reported values determined for the same proteins based on their responses to different ionic environments.
机译：在含有0.5 M蔗糖或0.5 M海藻糖的葡聚糖-PEG-磷酸钠/磷酸钾水溶液（0.01 M K / NaPB，pH 7.4）两相系统（ATPS）中检查了11种不同蛋白质和30种有机化合物的分配。将获得的数据与先前报道的相同化合物和蛋白质在不含渗透压的ATPS和含0.5 M TMAO的ATPS中的数据进行比较（Breydo等人（2015年），生物化学和生物物理学档案馆，印刷中），并根据所谓Collander线性溶剂回归关系。发现存在0.5M蔗糖和海藻糖时蛋白质分配系数的对数线性相关。估计蛋白质3D结构相对于核糖核酸酶B的结构距离。这些估计值显示与先前报告的值线性相关，这些值是根据相同蛋白质对不同离子环境的反应确定的。

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