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Simplified aptamer-based colorimetric method using unmodified gold nanoparticles for the detection of carcinoma embryonic antigen

机译：使用未修饰的金纳米粒子的基于适体的简化比色法，用于检测癌胚抗原

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摘要

Here, we described a simple and sensitive colorimetric method for the detection of carcinoma embryonic antigen (CEA), which was based on the phenomenon of salt-induced gold nanoparticles (AuNPs) aggregation and the conformation change of CEA's single-stranded DNA aptamer modified with a sulfhydryl group. Because of the reaction between the thiol group and AuNPs, the aptamer could bind to the surface of AuNPs strongly. AuNPs modified with aptamer had good stability in a high concentration of saline solution because of the repulsion of negative charges among the aptamers. In the presence of CEA, the aptamer tended to form a CEA-aptamer duplex instead of binding to the surface of AuNPs. As a result, the AuNPs changed its color from red to blue easily due to the salt-induced aggregation in the presence of CEA. Accordingly, the CEA was detected using this method. By adjusting the amount of the aptamer and NaCl added, a sensitive linear range for CEA was obtained. Under the optimum conditions, the detection limit for CEA was 3 ng mL(-1). This rapid, label-free assay using colorimetric method showed high sensitivity for CEA detection, which made it a practical way in some cancer biomarker detections.
机译：在这里，我们描述了一种简单而灵敏的比色法，用于检测癌胚抗原（CEA），该方法基于盐诱导的金纳米颗粒（AuNPs）聚集现象和经修饰的CEA单链DNA适体的构象变化。巯基。由于硫醇基团与AuNPs之间的反应，适体可以牢固地结合到AuNPs的表面。适体修饰的AuNPs在高浓度盐溶液中具有良好的稳定性，因为适体之间排斥负电荷。在CEA的存在下，适体倾向于形成CEA-适体双链体而不是结合至AuNPs的表面。结果，由于在CEA存在下盐诱导的聚集，AuNPs的颜色容易从红色变为蓝色。因此，使用该方法检测到CEA。通过调节适体和NaCl的添加量，获得了CEA的灵敏线性范围。在最佳条件下，CEA的检出限为3 ng mL（-1）。这种使用比色法的快速，无标记的测定方法对CEA检测具有很高的灵敏度，这使其成为某些癌症生物标记物检测的实用方法。

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