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A porous hybrid imprinted membrane for selectively anchoring target proteins from a complex matrix

机译：多孔杂交印迹膜，用于选择性锚定复杂基质中的目标蛋白

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摘要

A novel porous hybrid imprinted membrane (CP/CNT/DA-MIM) was prepared that could selectively anchor and separate target proteins from a complex matrix. CP/CNT/DA-MIM exhibits many of the advantages of molecularly imprinted polymers and membranes, including the high selectivity of MIPs, the lower energy consumption and the ability to continuously separate mixtures via membrane separation. The surface morphologies and physical/chemical properties of the different membranes were investigated using FTIR, XRD, DSC, XPS and SEM. The results showed that the different molecules contained within CP/CNT/DA-MIM were homogeneous; two different sizes of imprinted cavities were observed in CP/CNT/DA-MIM, which facilitate the selective anchoring property. The adsorption capacities, swelling behaviors and mechanical properties of the different constituent membranes were also compared. The results show that the adhesion and nonspecific adsorption properties of the membrane were manifestly reduced through the addition of PVP. The binding capacity and adsorption selectivity of the membrane were apparently improved because of the presence of dopamine. MWCNTs obviously improved the mechanical strength of the membranes. CP/CNT/DA-MIM was successfully applied to separate bovine serum albumin from bovine blood. CP/CNT/DA-MIM is an economical, hydrophilic and ecofriendly membrane and is a promising separation material for the large-scale continuous selective separation of target proteins from complex matrices in commercial applications.
机译：制备了一种新型的多孔杂种印迹膜（CP / CNT / DA-MIM），可以选择性地从复杂基质中锚定和分离目标蛋白。 CP / CNT / DA-MIM具有分子印迹聚合物和膜的许多优点，包括MIP的高选择性，较低的能耗以及通过膜分离连续分离混合物的能力。使用FTIR，XRD，DSC，XPS和SEM研究了不同膜的表面形态和物理/化学性质。结果表明，CP / CNT / DA-MIM中包含的不同分子是均质的。在CP / CNT / DA-MIM中观察到两种不同大小的压印腔，这有助于选择性锚固性能。还比较了不同组成膜的吸附能力，溶胀行为和机械性能。结果表明，通过添加PVP，膜的粘附力和非特异性吸附性能明显降低。由于多巴胺的存在，膜的结合能力和吸附选择性明显提高。 MWCNTs明显提高了膜的机械强度。 CP / CNT / DA-MIM已成功应用于从牛血中分离牛血清白蛋白。 CP / CNT / DA-MIM是一种经济，亲水和环保的膜，是一种有前途的分离材料，可用于在商业应用中从复杂基质大规模连续选择性地分离目标蛋白。

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