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首页> 外文期刊>RSC Advances >Aminomethylpyrene-based imino-phenols as primary fluorescence switch-on sensors for Al3+ in solution and in Vero cells and their complexes as secondary recognition ensembles toward pyrophosphate
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Aminomethylpyrene-based imino-phenols as primary fluorescence switch-on sensors for Al3+ in solution and in Vero cells and their complexes as secondary recognition ensembles toward pyrophosphate

机译：基于氨基甲基py的亚氨基酚作为溶液和Vero细胞中Al3 +的主要荧光开关传感器，并且作为次级识别的复合物朝向焦磷酸盐形成

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摘要

Three aminomethylpyrene-based salicyl-imines, viz. L1, L2 and L3 were synthesized and characterized and their recognition of biologically relevant Mn+ ions was studied. These three receptors were shown to be selective and sensitive for Al3+ among the 13 metal ions studied in a HEPES buffer medium by fluorescence, absorption, and visual emission color change with detection limits of 3.60, 2.13 and 2.16 mu M, respectively, by L1, L2 and L3. The interaction of Al3+ with the three receptors (L1, L2 and L3) has been further supported by absorption studies, and the stoichiometry of the complex formed (1 : 1) has been established on the basis of emission and ESI-MS. Competitive ion titrations carried out reveal that the Al3+ can be detected even in the presence of other metal ions of bio importance. The structure of the aluminium complexes and their mode of interactions were established by DFT calculations. TDDFT calculations were performed in order to demonstrate the electronic properties of receptors. Microstructural features of L2 and its Al3+ complex have been measured by AFM. Moreover, the utility of the receptors L1, L2 and L3 in showing aluminium recognition in live cells has also been demonstrated using Vero cells as monitored by fluorescence imaging. In situ prepared [AlL1] and [AlL3] complexes were found to be sensitive and selective toward phosphate-bearing ions and molecules and in particular to pyrophosphate (PPi) among the other 15 anions studied; however, [AlL2] was not sensitive toward any of the anions studied.
机译：三个基于氨基甲基py的水杨基亚胺，即。合成并表征了L1，L2和L3，并研究了它们对生物学相关的Mn +离子的识别。在HEPES缓冲液中研究的13种金属离子中，这三种受体对Al3 +具有选择性和敏感性，通过L1，L1的荧光，吸收和可见发射颜色变化分别检测出3.60、2.13和2.16μM的铝离子， L2和L3。吸收研究进一步支持了Al3 +与三种受体（L1，L2和L3）的相互作用，并基于发射和ESI-MS建立了所形成的配合物（1：1）的化学计量。进行竞争性离子滴定表明，即使存在其他具有生物重要性的金属离子，也可以检测到Al3 +。通过DFT计算确定了铝配合物的结构及其相互作用方式。为了证明受体的电子性质，进行了TDDFT计算。 L2及其Al3 +配合物的微结构特征已通过AFM测量。此外，还使用Vero细胞证明了受体L1，L2和L3在活细胞中显示铝识别的效用，如荧光成像所监测的那样。发现原位制备的[AlL1]和[AlL3]配合物对含磷酸盐的离子和分子特别是对焦磷酸盐（PPi）敏感，对其他15种阴离子具有选择性。但是，[AlL2]对所研究的任何阴离子都不敏感。

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