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首页> 外文期刊>RSC Advances >Surface charge effect on the cellular interaction and cytotoxicity of NaYF4:Yb3+, Er3+@SiO2 nanoparticles
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Surface charge effect on the cellular interaction and cytotoxicity of NaYF4:Yb3+, Er3+@SiO2 nanoparticles

机译：表面电荷对NaYF4：Yb3 +，Er3 + @ SiO2纳米颗粒的细胞相互作用和细胞毒性的影响

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摘要

Taking advantage of the unique optical properties of upconversion nanoparticles (NP) and the feasibly modified nature of SiO2, NaYF4:Yb3+, Er3+@SiO2 core@shell (UCNP@SiO2) NPs with excellent morphology have been synthesized to investigate the surface charge effect on the interactions of NPs with cells. The cellular uptake behaviors, cytotoxicities and intracellular metal ion release of the as-prepared NPs have been investigated in two cell lines (HeLa and HepG-2) by fluorescence microscopy, MTT assay and inductively coupled plasma mass spectrometry (ICP-MS), respectively. It is found that the UCNP@SiO2 NPs with positive surface charge have much higher cell internalization ability and cytotoxicity than those with negative surface charge. The experimental results also demonstrate that the cytotoxicity of UCNP@SiO2 NP is closely related to the increase of intracellular reactive oxygen species (ROS) and metal ion release of internalized NPs.
机译：利用上转换纳米粒子（NP）的独特光学性质和SiO2的适度改性性质，合成了具有优异形态的NPs NaYF4：Yb3 +，Er3 + @ SiO2核@壳（UCNP @ SiO2）NP，以研究其表面电荷效应。 NP与细胞的相互作用。通过荧光显微镜，MTT测定和电感耦合等离子体质谱法（ICP-MS）分别在两种细胞系（HeLa和HepG-2）中研究了制备的NP的细胞吸收行为，细胞毒性和细胞内金属离子释放。发现具有正表面电荷的UCNP @ SiO2 NPs具有比具有负表面电荷的UCNP @ SiO2 NPs高得多的细胞内在化能力和细胞毒性。实验结果还表明，UCNP @ SiO2 NP的细胞毒性与细胞内活性氧（ROS）的增加和内在化NP的金属离子释放密切相关。

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