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A SERS study of oxidation of glutathione under plasma irradiation

机译：等离子体照射下谷胱甘肽氧化的SERS研究

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This paper reports a new application of surface enhanced Raman scattering (SERS) in analysis of oxidation of glutathione (GSH) to oxidized glutathione (GSSG), an important biochemical redox reaction in biological systems, under oxidative stress imposed by dielectric barrier discharge (DBD). Using the silver nanoparticles (NPs) prepared through the reduction of AgNO3 by beta-cyclodextrin (beta-CD), the transformation of GSH to GSSG under DBD irradiation can be probed with only a small quantity of sample at low concentration. Based on the intensity ratio of two characteristic Raman bands, i.e., the band at 1051 cm(-1) (C-N stretching) and the band at 509 cm(-1) (S-S stretching), which stem respectively from GSH and GSSG, the conversion between the reduced and oxidized glutathione can be determined quantitatively. This work demonstrates another useful extension of the SERS technique applied to bioscience research, i.e., rapid probing and quantitative assessing of chemical reactions of biomolecules under oxidative stress conditions.
机译：本文报道了表面增强拉曼散射（SERS）在谷胱甘肽（GSH）氧化为氧化型谷胱甘肽（GSSG）的分析中的新应用，这是生物系统中重要的生化氧化还原反应，在介电势垒放电（DBD）施加的氧化应力下。使用通过β-环糊精（β-CD）还原AgNO3制备的银纳米颗粒（NPs），仅用少量低浓度样品即可探测DBD照射下GSH向GSSG的转化。根据两个特征拉曼谱带的强度比，即分别来自GSH和GSSG的1051 cm（-1）处的谱带（CN拉伸）和509 cm（-1）处的谱带（SS拉伸），还原型和氧化型谷胱甘肽之间的转化率可以定量确定。这项工作证明了用于生物科学研究的SERS技术的另一个有用扩展，即在氧化应激条件下快速探测和定量评估生物分子的化学反应。

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