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首页> 外文期刊>RSC Advances >Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity
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Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity

机译：使用葵花籽油皂料从克劳氏芽孢杆菌BS02提取脂生物表面活性剂：高通量筛选方法，生产，纯化，表征及其杀虫活性的评价

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摘要

A total of 76 bacterial isolates were screened for biosurfactant activity using 16 different high throughput screening methods. Based on phenotypic characteristics, 16S rDNA sequencing and FAME profiling, isolate BS02, identified as Bacillus clausii BS02 produced a biosurfactant (2.6 g l(-1)) when grown in basal salts mineral medium with sunflower oil soapstock (3% w/v) at 30 degrees C and 120 rpm after 42 h. The cell free broth showed 82% emulsification activity, critical micelle concentration of 45 mg l(-1) and reduced the surface tension from 69.07 mN m(-1) to 30 mN m(-1). Acid precipitated and methanol extracted crude biosurfactant had 64.3%, 28%, and 4.5% (w/w) of protein, lipid, and carbohydrate, respectively. The crude biosurfactant exhibited stability with oils, fats and non-aqueous phase liquids. Fourier transform infrared (FT-IR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), and nuclear magnetic resonance spectroscopy (NMR) revealed the functional groups and bonds; X-ray diffraction (XRD) and thermogravimetry (TG) showed the surface nature and thermostability of the biosurfactant, respectively. LC-ESI-MS identified the crude biosurfactant as a surfactin lipopeptide. A dose-dependent mortality against C. chinensis and M. hirsutus was observed using the crude biosurfactant, with a LC50 of 50 mu g ml(-1), indicating the first evidence of its insecticidal activity against pulse beetles and mealybugs.
机译：使用16种不同的高通量筛选方法筛选了76种细菌分离株的生物表面活性剂活性。基于表型特征，16S rDNA测序和FAME分析，分离株BS02（鉴定为克劳氏芽孢杆菌BS02）在具有向日葵油皂液（3％w / v）的基础盐矿物培养基中生长时，可产生生物表面活性剂（2.6 gl（-1）） 42小时后，在30摄氏度和120 rpm下进行。无细胞肉汤显示出82％的乳化活性，临界胶束浓度为45 mg l（-1），表面张力从69.07 mN m（-1）降低到30 mN m（-1）。酸沉淀和甲醇萃取的粗生物表面活性剂分别具有64.3％，28％和4.5％（w / w）的蛋白质，脂质和碳水化合物。粗生物表面活性剂对油，脂肪和非水相液体表现出稳定性。傅立叶变换红外（FT-IR）光谱，能量色散X射线光谱（EDX）和核​​磁共振光谱（NMR）揭示了官能团和键。 X射线衍射（XRD）和热重分析（TG）分别显示了生物表面活性剂的表面性质和热稳定性。 LC-ESI-MS将粗制生物表面活性剂鉴定为表面活性素脂肽。使用粗生物表面活性剂观察到的针对中华绒螯蟹和多毛念珠菌的剂量依赖性死亡率，LC50为50μg ml（-1），这表明了其对脉冲甲虫和粉虱的杀虫活性。

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