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首页> 外文期刊>RSC Advances >Enzymatic delignification: an attempt for lignin degradation from lignocellulosic feedstock
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Enzymatic delignification: an attempt for lignin degradation from lignocellulosic feedstock

机译：酶法脱木素：尝试从木质纤维素原料中降解木质素

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摘要

Burgeoning population growth and an increased demand for transportation and industrialization has led to the excessive use of fossil fuels, which in turn leads to higher levels of greenhouse gas emissions and contributes to global warming. At this juncture, biomass-based biofuel production from sustainable resources such as lignocellulosics acts as a better alternative for achieving zero emissions. This in turn necessitates the importance of development of an efficient biomass delignification method, which is an essential prerequisite for the complete biofuel production process. Lignocellulosics such as Saccharum spontaneum contain 17.46% of lignins and 67% of carbohydrates within its cell walls. To make this enormous amount of carbohydrates more accessible for hydrolysis and for further use in fermentation, lignin degradation through laccase has been carried out. In the present study, response surface methodology (RSM) based on central composite design (CCD) has been used to investigate the effects of the various process parameters. The maximum delignification obtained was 84.67% at 6.21 h of incubation time upon monitoring the initial lignin content of 17.46% of the biomass. Thorough study of the biomass was carried out by elemental composition analysis and energy density measurement. Further structural characteristics of the delignified substrate were analyzed by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) spectroscopy, which supported the efficacy of the delignification process.
机译：迅速增长的人口增长以及对运输和工业化需求的增长，导致了对化石燃料的过度使用，进而导致温室气体排放量增加，并助长了全球变暖。在此关头，利用可持续资源（如木质纤维素）生产基于生物质的生物燃料可作为实现零排放的更好选择。这进而需要开发有效的生物质脱木素方法的重要性，这是完整生物燃料生产过程的必要先决条件。木质纤维素，例如自然发芽糖，在其细胞壁中含有17.46％的木质素和67％的碳水化合物。为了使大量碳水化合物更易于水解和进一步用于发酵，已经通过漆酶降解木质素。在本研究中，基于中央复合设计（CCD）的响应面方法（RSM）已用于研究各种工艺参数的影响。通过监测初始生物素含量为生物质的17.46％，在孵育时间6.21 h时获得的最大去木质素度为84.67％。通过元素组成分析和能量密度测量对生物质进行了深入研究。通过扫描电子显微镜（SEM），傅立叶变换红外（FTIR）光谱和X射线衍射（XRD）光谱分析了脱木素底物的其他结构特征，这证明了脱木素工艺的有效性。

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