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Enhanced enzymatic hydrolysis of lignocellulose by integrated decrystallization and fed-batch operation

机译：通过整合的去结晶和分批补料操作增强木质纤维素的酶促水解

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摘要

High solids enzymatic hydrolysis is a promising process to increase the concentrations of fermentable sugars for ethanol production. Here, we reported an integrated strategy incorporating decrystallization and fed-batch operation to enhance the enzymatic hydrolysis of lignocellulose at high solid loadings. The effect of concentration of phosphoric acid on the decrystallization and enzymatic hydrolysis of cellulose was investigated. The results showed that 86 wt% phosphoric acid could completely break the crystalline structure, achieving a high cellulose conversion and hydrolysis rate (89% within 4 h) in batch operation (5% solid loading). In the fed-batch operation, we designed two different enzyme feeding strategies, in which the enzymes were added either at start-up or along with the fresh substrate. The results indicated that fed-batch hydrolysis can be carried out at a final high solids loading of 30%, resulting in a glucose concentration as high as 143.5 g L-1 within 27 hours. Meanwhile, a stable and high cellulose conversion was achieved in fed-batch operation with the enzymes divided over the substrate feed, which should be dependent on the decrystallization of the substrate. Our findings provide a practical way to enhance enzymatic hydrolysis at high solid loadings and also propose a feasible enzyme feeding strategy in fed-batch operation.
机译：高固体酶水解是增加乙醇生产用可发酵糖浓度的一种有前途的方法。在这里，我们报道了一种整合策略，该策略结合了去结晶和补料分批操作，以提高木质素纤维素在高固体含量下的酶促水解。研究了磷酸浓度对纤维素脱结晶和酶促水解的影响。结果表明，在批处理操作（固体含量为5％）下，86 wt％的磷酸可以完全破坏晶体结构，实现较高的纤维素转化率和水解率（4 h内为89％）。在分批补料操作中，我们设计了两种不同的酶补料策略，其中在启动时或与新鲜底物一起添加酶。结果表明，分批补料水解可以在最终的高固体含量为30％的条件下进行，从而使葡萄糖浓度在27小时内高达143.5 g L-1。同时，在分批补料操作中获得了稳定且高的纤维素转化率，其中酶分配在底物进料上，这应取决于底物的去结晶。我们的发现提供了一种在高固体含量下增强酶水解的实用方法，并且提出了分批补料操作中可行的酶补料策略。

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