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首页> 外文期刊>RSC Advances >A sandwich electrochemical immunoassay for Salmonella pullorum and Salmonella gallinarum based on a AuNPs/SiO2/Fe3O4 adsorbing antibody and 4 channel screen printed carbon electrode electrodeposited gold nanoparticles
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A sandwich electrochemical immunoassay for Salmonella pullorum and Salmonella gallinarum based on a AuNPs/SiO2/Fe3O4 adsorbing antibody and 4 channel screen printed carbon electrode electrodeposited gold nanoparticles

机译：基于AuNPs / SiO2 / Fe3O4吸附抗体和4通道丝网印刷碳电极电沉积金纳米颗粒的鸡白痢沙门氏菌和鸡沙门氏菌的夹心电化学免疫分析

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A rapid and highly sensitive sandwich electrochemical immunoassay method was constructed for Salmonella pullorum and Salmonella gallinarum (S. pullorum and S. gallinarum) determination based on immune magnetic beads (MBs) and an enzyme labeled antibody. An abundance of gold nanoparticles (AuNPs) were attached to SiO2 coated Fe3O4 nanoparticles (Fe3O4/SiO2) via covalent binding between the -SH groups of Fe3O4/SiO2 and the AuNPs. Antibodies against S. pullorum and S. gallinarum were immobilized on Fe3O4/SiO2/AuNPs nanocomposites (AuMNPs) by automatic adsorption between thiol and the AuNPs. S. pullorum and S. gallinarum in the sample were captured by the AuMNPs and separated from the samples by applying an external magnetic field. The AuMNPs-Salmonella complexes (Ag/Ab(1)/AuMNPs) were re-dispersed in a buffer solution then exposed to horseradish peroxidase labeled anti-S. pullorum and S. gallinarum (HRP-Ab(2)) solution, forming a sandwich-type immune complex (HRP-Ab(2)/Ag/Ab(1)/AuMNPs). A 4 channel screen printed carbon electrode (4-SPCE) was modified by gold nanoparticles (AuNPs) through the electrodeposition method to prepare AuNPs/4-SPCE. After magnetically separating the sandwich immune complexes from solution, HRP-Ab(2)/Ag/Ab(1)/AuMNPs was anchored on AuNPs/4-SPCE by a magnet. A linear response to S. pullorum and S. gallinarum was obtained in the concentration range from 10(2) to 10(6) CFU mL(-1), with a limit of detection of 3.2 x 10(1) CFU mL(-1) (at an SNR of 3). This nanoparticle-based immunoassay method offers sensitive, highly specific, and reproducible detection of S. pullorum and S. gallinarum. Given its low detection limit, it represents promising potential in the detection of other food-borne pathogens by exchanging the antibody.
机译：建立了一种快速，高灵敏度的夹心电化学免疫分析方法，用于基于免疫磁珠（MBs）和酶标记的抗体测定沙门氏菌和鸡沙门氏菌（鸡痢疾沙门氏菌和鸡沙门氏菌）。大量的金纳米粒子（AuNPs）通过Fe3O4 / SiO2的-SH基团与AuNPs之间的共价结合而附着在SiO2包覆的Fe3O4纳米粒子（Fe3O4 / SiO2）上。通过在硫醇和AuNP之间的自动吸附，将针对白痢链球菌和鸡沙门氏菌的抗体固定在Fe3O4 / SiO2 / AuNPs纳米复合材料（AuMNPs）上。样品中的白痢链球菌和鸡毒链霉菌被AuMNP捕获，并通过施加外部磁场与样品分离。将AuMNPs-沙门氏菌复合物（Ag / Ab（1）/ AuMNPs）重新分散在缓冲溶液中，然后暴露于辣根过氧化物酶标记的抗S物质。鸡白痢和鸡沙门氏菌（HRP-Ab（2））溶液，形成三明治型免疫复合物（HRP-Ab（2）/ Ag / Ab（1）/ AuMNPs）。采用金纳米颗粒（AuNPs）通过电沉积方法对4通道丝网印刷碳电极（4-SPCE）进行修饰，制备出AuNPs / 4-SPCE。从溶液中磁性分离夹心免疫复合物后，用磁铁将HRP-Ab（2）/ Ag / Ab（1）/ AuMNPs固定在AuNPs / 4-SPCE上。在10（2）至10（6）CFU mL（-1）的浓度范围内，获得了对鸡白痢链球菌和鸡沙门氏菌的线性响应，检出限为3.2 x 10（1）CFU mL（- 1）（SNR为3）。这种基于纳米颗粒的免疫测定方法提供灵敏，高度特异性和可重现的白痢链球菌和鸡链球菌的检测。鉴于其低检测限，它通过交换抗体在检测其他食源性病原体方面具有广阔的潜力。

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