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首页> 外文期刊>RSC Advances >Highly efficient colorimetric detection of ATP utilizing a split aptamer target binding strategy and superior catalytic activity of graphene oxide-platinum/gold nanoparticles
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Highly efficient colorimetric detection of ATP utilizing a split aptamer target binding strategy and superior catalytic activity of graphene oxide-platinum/gold nanoparticles

机译：利用分离的适体靶标结合策略高效地比色检测ATP和氧化石墨烯-铂/金纳米颗粒的出色催化活性

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摘要

In this study, a highly efficient colorimetric assay for adenosine triphosphate (ATP) was constructed based on a split aptamer target binding strategy and grapheme oxide-platinum/gold nanoparticle platform to increase catalytic properties. Platinum/gold nanoparticles were easily prepared by reducing the mixture solution of K2PtCl6 and HAuCl4 using sodium citrate in a one-pot synthesis. Next, graphene oxide/ platinum/gold nanoparticle hybrids (GO/PDDA/PtAuNPs) were fabricated using a self-assembly procedure. Ultrasensitive and enzyme-free detection of ATP in fetal bovine serum was achieved using split aptamer-modified magnetic beads as a separating unit and split aptamer-modified GO/PDDA/PtAuNPs as a catalyst-detecting platform. The specific binding of ATP and its aptamer linked the split aptamer-modified GO/PDDA/PtAuNPs and magnetic beads together. Using magnetic separation, achromatous tetramethylbenzidine was catalyzed into a colored product by separated nanocomposites, which enabled rapid detection of ATP. The color change caused by the low concentration ATP (50 nM) was clearly distinguished by direct observation. The detection limit of the colorimetric assay for ATP was 0.2 nM, which is close to the lowest detection limit for colorimetric detection of ATP. This assay showed excellent selectivity against guanosine triphosphate, uridine triphosphate, and cytosine triphosphate and can be applied in real samples.
机译：在这项研究中，基于分裂的适体靶标结合策略和氧化石墨-铂/金纳米颗粒平台构建了高效的比色法测定三磷酸腺苷（ATP），以提高催化性能。通过在一锅合成中使用柠檬酸钠还原K2PtCl6和HAuCl4的混合溶液，可以轻松制备铂/金纳米颗粒。接下来，使用自组装程序制造氧化石墨烯/铂/金纳米颗粒杂化物（GO / PDDA / PtAuNPs）。使用分裂的适体修饰的磁珠作为分离单元，以及分裂的适体修饰的GO / PDDA / PtAuNPs作为催化剂检测平台，实现了胎牛血清中ATP的超灵敏和无酶检测。 ATP及其适体的特异性结合将分裂的适体修饰的GO / PDDA / PtAuNP和磁珠连接在一起。使用磁分离，无色四甲基联苯胺被分离出的纳米复合物催化成有色产物，从而可以快速检测ATP。通过直接观察可以清楚地区分低浓度ATP（50 nM）引起的颜色变化。 ATP的比色测定的检测下限为0.2 nM，接近ATP的比色检测的最低检测下限。该测定法显示出对三磷酸鸟苷，三磷酸尿苷和三磷酸胞嘧啶具有极好的选择性，可用于实际样品中。

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