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Scale-up of removal process using a remediating-bacterium isolated from marine coastal sediment

机译:使用从海洋沿海沉积物中分离出来的修复细菌扩大去除过程的规模

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Nowadays, a wide variety of pollutants are discharged to different water sources and become water contaminants. To overcome this problem, bioremediation has been studied as an alternative for their treatment due to its low cost, high efficiency and environmentally friendliness. However, it is necessary to search for microorganisms able to remediate pollutants of different nature. In this work, the isolation and identification of remediating-bacteria from polluted marine coastal sediment were carried out. The isolation assays were carried out using phenanthrene as the only carbon source. The identification of the strains was performed by PCR amplification of 16S rDNA. It was resolved in one band and the sequencing showed that this band was derived from Serratia plymuthica. The removal ability of this microorganism was assayed with phenanthrene, benzo[a] pyrene, different insecticides and Cr(VI). The isolated bacterium showed high removal ability of imidacloprid, methomyl, fenamiphos, and Cr(VI). After that, bioreactor scale-up (5 L) was studied with a mixture of these pollutants. Total removal degrees were obtained for organic compounds and around 96% of Cr(VI) removal was reached. Furthermore, the removal rate of the different pollutants fitted well to a logistic model. The present study states that the identified bacterium can play a vital role in bioremediation of an aquatic environment polluted with mixtures of contaminants.
机译:如今,各种各样的污染物被排放到不同的水源并成为水污染物。为了克服这个问题,由于其低成本,高效率和环境友好性,已经研究了生物修复作为其处理的替代方法。但是,有必要寻找能够修复不同性质污染物的微生物。在这项工作中,从污染的海洋沿海沉积物中分离和鉴定了修复细菌。分离测定使用菲作为唯一碳源进行。通过PCR扩增16S rDNA进行菌株鉴定。它被解析为一个条带,测序表明该条带来源于粘质沙雷氏菌。用菲,苯并[a] pyr,不同的杀虫剂和六价铬(VI)测定了该微生物的去除能力。分离出的细菌显示出吡虫啉,灭多威,苯胺磷和Cr(VI)的高去除能力。之后,研究了使用这些污染物的混合物进行生物反应器放大(5 L)的过程。获得了有机化合物的总去除度,并且达到了约96%的Cr(VI)去除率。此外,不同污染物的去除率非常适合逻辑模型。本研究表明,鉴定出的细菌在污染混合物污染的水生环境的生物修复中起着至关重要的作用。

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