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首页> 外文期刊>RSC Advances >An integrated metabonomics and microbiology analysis of host-microbiota metabolic interactions in rats with Coptis chinensis-induced diarrhea
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An integrated metabonomics and microbiology analysis of host-microbiota metabolic interactions in rats with Coptis chinensis-induced diarrhea

机译：黄连致腹泻大鼠宿主-菌群代谢相互作用的代谢组学和微生物学分析

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摘要

Coptis chinensis Franch., a bererine-containing traditional Chinese medicine (TCM), is often used to treat intestinal infections, diabetes and hyperlipidaemia, and often causes diarrhea. To clarify the potential mechanism of toxicity that induces diarrhea, Sprague-Dawley (SD) rats were treated with Coptis chinensis dosage of 5 g kg(-1) for 14 consecutive days. PCR-denaturing gradient gel electrophoresis (PCR-DGGE) was used to monitor the dynamic changes in the gut microbiota, while H-1 NMR profiles were applied to reveal the metabolism of host and microflora. In the Coptis chinensis-treated group, decreased short chain fatty acids (SCFAs) and branched chain fatty acids (BCFAs) and increased branched chain amino acids (BCAAs) levels were detected in faeces, whereas increased BCFAs were present in the urine. This finding implied that Coptis chinensis triggered malabsorption and suppressed bacterial fermentation as well as protein degradation. Meanwhile, decreased levels of Bacteroides and Prevotella and elevated levels of Enterobacter and Veillonella in the treatment group were significantly correlated to the urinary and faecal metabolites. Using metabolite-set enrichment analysis (MSEA) and the correlation analysis between significant bacteria and metabolites, the results demonstrated that Coptis chinensis intervention suppressed glycine and serine metabolism which affected the growth of intestinal bacteria. Moreover, the perturbed microbiome consequently influenced the homeostasis of monosaccharides, amino acids, and choline, and energy metabolism of gut microbiota and host. These findings help to elucidate Coptis chinensis intervention and toxicity; simultaneously, this integrated strategy may provide an effective method for the systematic assessment of host responses to TCM or any other botanical-based nutraceuticals.
机译：黄连，一种含有铍的中药（TCM），通常用于治疗肠道感染，糖尿病和高脂血症，并经常引起腹泻。为了阐明引起腹泻的潜在毒性机制，连续5天以5 g kg（-1）的黄连剂量治疗Sprague-Dawley（SD）大鼠。 PCR-变性梯度凝胶电泳（PCR-DGGE）用于监测肠道菌群的动态变化，而H-1 NMR谱用于揭示宿主和菌群的代谢。在黄连治疗组中，粪便中短链脂肪酸（SCFA）和支链脂肪酸（BCFA）的含量降低，而支链氨基酸（BCAAs）的含量升高，而尿液中的BCFA含量升高。这一发现暗示黄连引发了吸收不良并抑制了细菌发酵以及蛋白质降解。同时，治疗组中的拟杆菌属和普氏杆菌属水平降低以及肠杆菌和韦永氏菌水平升高与尿液和粪便代谢产物显着相关。使用代谢物组富集分析（MSEA）以及重要细菌与代谢物之间的相关性分析，结果表明黄连干预抑制了甘氨酸和丝氨酸代谢，从而影响肠道细菌的生长。此外，受干扰的微生物组因此影响了单糖，氨基酸和胆碱的稳态，以及肠道菌群和宿主的能量代谢。这些发现有助于阐明黄连的干预作用和毒性。同时，这种综合策略可能为系统评估宿主对中药或其他植物性保健食品的反应提供有效的方法。

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