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首页> 外文期刊>RSC Advances >Influence of photoactivated tetra sulphonatophenyl porphyrin and TiO2 nanowhiskers on rheumatoid arthritis infected bone marrow stem cell proliferation in vitro and oxidative stress biomarkers in vivo
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Influence of photoactivated tetra sulphonatophenyl porphyrin and TiO2 nanowhiskers on rheumatoid arthritis infected bone marrow stem cell proliferation in vitro and oxidative stress biomarkers in vivo

机译：光活化四磺基苯基卟啉和TiO2纳米晶须对类风湿关节炎感染的骨髓干细胞的体外增殖及体内氧化应激生物标志物的影响

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Photodynamic therapy (PDT) is mostly used to induce apoptosis or necrosis in benign and malignant tumors, along with other microbial infections and suppression of autoimmune diseases including rheumatoid arthritis (RA). The bone marrow stem (BMS) cells are also a focus in translational medicine, tissue engineering and as an autoimmune disease suppressant. In this study we used tetra sulphonatophenyl porphyrin (TSPP) with TiO2 nanowhiskers for RA PDT and evaluated the effect on stress biomarkers (CAT, SOD, GPX, GR, TAO and MDA) in vivo and BMS cell proliferation in vitro. We compared four murine groups, three of which had collagen induced arthritis as TP-L (illuminated), TP-nL (dark) and CIA (control), whereas the other group was normal without disease and treatment. All anti-oxidative enzymes and biomarkers were significantly (p < 0.01) affected by the treatment except TAO (p > 0.05). Moreover, we also evaluated the growth proliferation effect of TSPP-TiO2 (TP) PDT on the in vitro RA infected BMS cells i.e. 25 mu l had highest cell count (12.33 x 10(6) cells per well) and 33% higher growth rate in photoactivated TP when compared with 50 and 100 mu l treatment groups. Herein, we report that photoactivated TSPP-TiO2 for RA PDT may be safer than photosensitizers without the titanium nanomaterials in terms of reduced oxidative stress and also promotion of RA BMS cell growth in vitro as a novel finding.
机译：光动力疗法（PDT）主要用于诱导良性和恶性肿瘤的凋亡或坏死，以及其他微生物感染和抑制包括类风湿关节炎（RA）在内的自身免疫性疾病。骨髓干（BMS）细胞也是转化医学，组织工程学和自身免疫性疾病抑制剂的重点。在这项研究中，我们将四磺基苯基卟啉（TSPP）与TiO2纳米晶须用于RA PDT，并评估了其对体内应激生物标记（CAT，SOD，GPX，GR，TAO和MDA）和体外BMS细胞增殖的影响。我们比较了四个鼠类，其中三个具有胶原蛋白诱发的关节炎，如TP-L（亮），TP-nL（暗）和CIA（对照组），而另一组则正常，没有疾病和治疗。除TAO（p> 0.05）外，所有抗氧化酶和生物标志物均受治疗的影响显着（p <0.01）。此外，我们还评估了TSPP-TiO2（TP）PDT对体外RA感染的BMS细胞的生长增殖作用，即25μl的细胞数最高（每孔12.33 x 10（6）个细胞），且生长速率高33％与50和100μl治疗组相比，光活化TP中的维生素C含量更高。在本文中，我们报道，对于RA PDT，光活化的TSPP-TiO2可能比不使用钛纳米材料的光敏剂更安全，这在减少氧化应激和促进RA BMS细胞体外生长方面是一个新发现。

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