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Integrated analysis of miRNA and mRNA expression profiles in development of porcine testes

机译：猪睾丸发育中miRNA和mRNA表达谱的综合分析

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To understand the complex physiological process underlying pig testis development and spermatogenesis, this study aims to characterize the change in miRNA and mRNA profiles at four developmental stages of embryonic and postnatal testes, including 60 dpc (days post coitus, E60), 90 dpc (E90), 30-day-old (D30) and 180-day-old (D180). A total of 304 mature, 50 novel miRNAs, and 8343 differentially-expressed genes were identified. 93 (48 up and 45 down), 104 (49 up and 55 down), 122 (49 up and 73 down) differentially-expressed miRNAs, as well as 1007 (646 up and 361 down), 1929 (911 up and 1018 down), 7420 (3998 up and 3422 down) differentially-expressed genes were identified in E90 vs. E60, D30 vs. E90 and D180 vs. D30, respectively. Integrating analysis of miRNA and mRNA expression profiles predicted more than 50 000 miRNA-mRNA interaction sites. GO and KEGG pathway analysis of the predicted target genes illustrated the likely roles of differentially expressed miRNAs in testis development and spermatogenesis. For example, PI3K-Akt signaling pathway and Hippo signaling pathway related development, and carbon metabolism, fatty acid metabolism, protein digestion and absorption, were involved in metabolite synthesis. These integrated high-throughput expression data show that miRNA is a critical factor in porcine testis development, providing a useful resource to understand global genome expression change in porcine testis development and spermatogenesis.
机译：为了了解猪睾丸发育和精子发生的复杂生理过程，本研究旨在表征胚胎和出生后睾丸四个发育阶段的miRNA和mRNA谱变化，包括60 dpc（性交后天数，E60），90 dpc（E90）。 ），30天（D30）和180天（D180）。总共确定了304个成熟的，50个新的miRNA和8343个差异表达的基因。 93（48向上和45向下），104（49向上和55向下），122（49向上和73向下）差异表达的miRNA，以及1007（646向上和361向下），1929（911向上和1018向下） ），分别在E90对E60，D30对E90和D180对D30中鉴定了7420个（3998向上和3422向下）差异表达的基因。对miRNA和mRNA表达谱的综合分析预测了5万多个miRNA-mRNA相互作用位点。 GO和KEGG通路的预测目标基因分析表明差异表达的miRNA在睾丸发育和生精中的可能作用。例如，PI3K-Akt信号通路和Hippo信号通路相关的发展，以及碳代谢，脂肪酸代谢，蛋白质的消化吸收，都参与了代谢产物的合成。这些综合的高通量表达数据表明，miRNA是猪睾丸发育的关键因素，为了解猪睾丸发育和精子发生的全球基因组表达变化提供了有用的资源。

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