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首页> 外文期刊>RSC Advances >Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism
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Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism

机译：混合不同真菌来源的纤维素分解酶制剂，通过增强协同作用改善纤维素水解

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摘要

Cellulolytic enzymes were produced from the three fungal strains [P. janthinellum EMS-UV-8 (E), T. reesei Rut C-30 (R) and A. tubingenesis (A)] and used to prepare blends for the hydrolysis of avicel and acid treated wheat straw (A-WS). An enzyme blend prepared from three different crude preparations (E + R + A) on the basis of equivalent FPU or protein was found to be more synergistic and gave better hydrolysis of avicel or A-WS than the blend of two enzyme preparations (E + R, E + A and R + A) or individual enzyme preparations (E, R, and A). The triple blend gave two times higher hydrolysis of avicel or A-WS than the individual enzyme preparations at the same enzyme dosages. In all cases the individual or cumulative FPU or protein in blends was equal (10 FPU or 20 mg protein per g of substrate). Increased enzyme activities (CMCase and FPU) were found in the blends compared to the sum of individual enzyme activities added for the blend preparation. This reveals that the increased hydrolysis of cellulose by blends was a result of increased synergism between the same (endoglucanase) and/or different types of cellulases from different preparations. Enzyme blending is thus a facile, cost effective and sustainable approach for biomass saccharification for biofuels.
机译：由三种真菌菌株产生纤维素分解酶[P.菊苣EMS-UV-8（E），里氏木霉Rut C-30（R）和油管曲霉（A）]，用于制备用于水解avicel和酸处理的小麦秸秆（A-WS）的混合物。发现由三种不同的粗制制剂（E + R + A）在同等的FPU或蛋白质的基础上制备的酶混合物比两种酶制剂的混合物（E + R，E + A和R + A）或单个酶制剂（E，R和A）。在相同的酶剂量下，三重共混物的avicel或A-WS水解度是单个酶制剂的两倍。在所有情况下，掺混物中的单个或累积FPU或蛋白质都是相同的（每克底物10 FPU或20 mg蛋白质）。与混合物制备中添加的各种酶活性的总和相比，混合物中的酶活性（CMCase和FPU）增加了。这表明掺混物增加的纤维素水解是相同的（内切葡聚糖酶）和/或来自不同制剂的不同类型的纤维素酶之间增效作用的结果。因此，酶掺合是一种简便，经济有效且可持续的生物燃料糖化糖化方法。

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