• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Reduced graphene oxide-titania based platform for label-free biosensor
【24h】

Reduced graphene oxide-titania based platform for label-free biosensor

机译：减少氧化石墨烯-二氧化钛的无标签生物传感器平台

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A label-free biosensor has been fabricated using a reduced graphene oxide (RGO) and anatase titania (ant-TiO2) nanocomposite, electrophoretically deposited onto an indium tin oxide coated glass substrate. The RGO-ant-TiO2 nanocomposite has been functionalized with protein (horseradish peroxidase) conjugated antibodies for the specific recognition and detection of Vibrio cholerae. The presence of Ab-Vc on the RGO-ant-TiO2 nanocomposite has been confirmed using electron microscopy, Fourier transform infrared spectroscopy and electrochemical techniques. Electrochemical studies relating to the fabricated Ab-Vc/RGO-ant-TiO2/ITO immunoelectrode have been conducted to investigate the binding kinetics. This immunosensor exhibits improved biosensing properties in the detection of Vibrio cholerae, with a sensitivity of 18.17 x 10(6) F mol(-1) L-1 m(-2) in the detection range of 0.12-5.4 nmol L-1, and a low detection limit of 0.12 nmol L-1. The association (k(a)), dissociation (k(d)) and equilibrium rate constants have been estimated to be 0.07 nM, 0.002 nM and 0.41 nM, respectively. This Ab-Vc/RGO-ant-TiO2/ITO immunoelectrode could be a suitable platform for the development of compact diagnostic devices.
机译：使用还原的氧化石墨烯（RGO）和锐钛型二氧化钛（ant-TiO2）纳米复合材料制造了无标记生物传感器，该材料通过电泳沉积在氧化铟锡涂层的玻璃基板上。 RGO-ant-TiO2纳米复合材料已与蛋白质（辣根过氧化物酶）偶联的抗体功能化，可特异性识别和检测霍乱弧菌。使用电子显微镜，傅立叶变换红外光谱和电化学技术已证实在RGO-ant-TiO2纳米复合材料上存在Ab-Vc。已经进行了与制备的Ab-Vc / RGO-ant-TiO2 / ITO免疫电极有关的电化学研究，以研究结合动力学。该免疫传感器在霍乱弧菌的检测中表现出改善的生物传感特性，在0.12-5.4 nmol L-1的检测范围内灵敏度为18.17 x 10（6）F mol（-1）L-1 m（-2），最低检测限为0.12 nmol L-1。关联（k（a）），解离（k（d））和平衡速率常数分别估计为0.07 nM，0.002 nM和0.41 nM。这种Ab-Vc / RGO-ant-TiO2 / ITO免疫电极可能是开发紧凑型诊断设备的合适平台。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号