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首页> 外文期刊>RSC Advances >Photophysical properties and in vitro cytotoxicity studies of new Ru(II) carbonyl complexes and mixed geometrical Ru(II)-Ni(II) complex in HS-DNA/BSA protein and human lung (A549) and liver (HepG2) cells
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Photophysical properties and in vitro cytotoxicity studies of new Ru(II) carbonyl complexes and mixed geometrical Ru(II)-Ni(II) complex in HS-DNA/BSA protein and human lung (A549) and liver (HepG2) cells

机译：HS-DNA / BSA蛋白和人肺（A549）和肝（HepG2）细胞中新型Ru（II）羰基配合物和混合几何Ru（II）-Ni（II）配合物的光物理性质和体外细胞毒性研究

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摘要

Two new ruthenium(II) carbonyl complexes have been synthesized from [RuHCl(CO)(PPh3)(3)] and 2-hydroxy-1-naphthaldehydethiosemicarbazone [H-2-(Nap-tsc)]. The stoichiometric reaction afforded two different complexes (1 and 2) exhibiting different structural motifs. In complex 1, the ligand coordinated through the N(2) nitrogen and thiolate sulphur by forming a strained four member chelate ring by leaving three donor sites (phenolic oxygen, N1 nitrogen and terminal nitrogen) uncoordinated. However, in complex 2, it coordinated as an ONS tridentate with the formation of a six member and a five member ring. In order to utilize the three unutilized donor sites in complex 1, it was reacted further with [NiCl2(PPh3)(2)], which resulted in the formation of a new hetero bimetallic complex 3 wherein all the donor atoms of the ligand were utilized. The complexes have been characterized analytically and spectroscopically and X-ray diffraction (1, 3) and have also been evaluated for their binding affinity with HS-DNA. The electrostatic binding of the complexes with DNA was evident from absorption and fluorescence titration experiments. The binding interaction between the complexes 1-3 and bovine serum albumin (BSA) was studied by absorption, fluorescence and synchronous spectra at room temperature. From the results, it is inferred that complex 2 had a better binding ability with the tryptophan residues of BSA. The mechanism of complex interaction was found as static quenching. The in vitro cytotoxicity of metal complexes (1-3) has been evaluated by colorimetric assay (MTT assay).
机译：从[RuHCl（CO）（PPh3）（3）]和2-羟基-1-萘乙硫基半脲[H-2-（Nap-tsc）]合成了两种新的钌（II）羰基配合物。化学计量反应提供了两种不同的复合物（1和2），它们具有不同的结构基序。在配合物1中，配体通过三个供体位点（酚氧，N1氮和末端氮）未配位而形成应变的四元螯合环，从而通过N（2）氮和硫醇盐硫进行配位。但是，在复合物2中，它作为ONS三齿形与六元环和五元环的形成相协调。为了利用络合物1中的三个未利用的供体位点，将其与[NiCl2（PPh3）（2）]进一步反应，从而形成新的杂双金属配合物3，其中利用了配体的所有供体原子。已通过分析和光谱学以及X射线衍射（1、3）对复合物进行了表征，并评估了它们与HS-DNA的结合亲和力。从吸收和荧光滴定实验中可以明显看出复合物与DNA的静电结合。在室温下通过吸收，荧光和同步光谱研究了配合物1-3与牛血清白蛋白（BSA）之间的结合相互作用。从结果推断，复合物2与BSA的色氨酸残基具有更好的结合能力。发现复杂相互作用的机理为静态淬灭。金属配合物（1-3）的体外细胞毒性已通过比色测定（MTT测定）进行了评估。

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