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The first example of a model compound of RNase U2 and its intermediate with CPP directly monitored by ESI-MS

机译:通过ESI-MS直接监测RNase U2及其中间体与CPP的模型化合物的第一个例子

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摘要

A Tb(III) complex of THP [1,4,7,10-tetrakis(2-hydroxypropyl)-cyclen] (THPTb) cleaved RNA phosphate ester bonds at A-specific sites to mimic RNase U2. The intermediates of the adducts of THPTb2+ and CPP- (cyclic propylene phosphate) or HPNP- (2-hydroxy propyl-p-nitrophenylphosphate) in the hydrolysis of HPNP were directly monitored by electrospray ionization mass spectrometry (ESI-MS).
机译:THP [1,4,7,10-四(2-羟丙基)-环蛋白](THPTb)的Tb(III)络合物在A特异性位点切割RNA磷酸酯键,以模拟RNase U2。通过电喷雾电离质谱法(ESI-MS)直接监测THPTb2 +与CPP-(环状磷酸丙烯酯)或HPNP-(2-羟基丙基-对硝基苯基磷酸酯)在HPNP水解中的加成中间体。

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