• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>RSC Advances >Simple approach for the immobilization of horseradish peroxidase on poly-L-histidine modified reduced graphene oxide for amperometric determination of dopamine and H2O2
【24h】

Simple approach for the immobilization of horseradish peroxidase on poly-L-histidine modified reduced graphene oxide for amperometric determination of dopamine and H2O2

机译：一种将辣根过氧化物酶固定在聚-L-组氨酸修饰的还原氧化石墨烯上的简单方法，用于安培法测定多巴胺和H2O2

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this work, immobilization of horseradish peroxidase (HRP) on poly-L-histidine (P-L-His) modified reduced graphene oxide (RGO) was demonstrated. The HRP/P-L-His-RGO bio-nanocomposite film was prepared through layer-by-layer (LBL) assembly. Scanning electron microscopy, Fourier transform infrared spectroscopy, electrochemical impedance spectroscopy, and UV-Vis spectroscopy were adopted to monitor the uniformity of the LBL assembly of the HRP/P-L-His-RGO bio-nanocomposite film. The immobilized HRP exhibited excellent electrocatalytic activity towards the reduction of hydrogen peroxide (H2O2). The catalysis currents showed a linear relationship with H2O2 concentration, ranging from 0.2 to 5000 mu M. The detection limit (S/N = 3) of H2O2 was 0.05 mu M. The apparent Michaelis-Menten constant (K-m) was calculated to be 1.2 mM. Moreover, the modified electrode displayed a rapid response (5 s) to H2O2 with good stability and reproducibility. Based on the HRP/P-L-His-RGO bio-nanocomposite film, a third-generation reagentless biosensor was constructed for the determination of H2O2.
机译：在这项工作中，已证明了辣根过氧化物酶（HRP）在聚L-组氨酸（P-L-His）修饰的氧化石墨烯（RGO）上的固定化。 HRP / P-L-His-RGO生物纳米复合膜是通过层（LBL）组装制备的。采用扫描电子显微镜，傅里叶变换红外光谱，电化学阻抗光谱和紫外可见光谱来监测HRP / P-L-His-RGO生物纳米复合膜LBL组件的均匀性。固定化的HRP对还原过氧化氢（H2O2）表现出优异的电催化活性。催化电流与H2O2浓度呈线性关系，范围为0.2至5000μM。H2O2的检出限（S / N = 3）为0.05μM。表观米氏（Michaelis-Menten）常数（Km）计算为1.2毫米此外，改性电极对H2O2表现出快速的响应（5 s），具有良好的稳定性和可重复性。基于HRP / P-L-His-RGO生物纳米复合膜，构建了用于测定H2O2的第三代无试剂生物传感器。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号