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首页> 外文期刊>RSC Advances >Magnetic electrode-based label-free electrochemical impedance spectroscopy immunosensor for sensitive detection of human malignant melanoma markers using gold nanoparticles functionalized magnetic graphene sheets as signal amplifier
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Magnetic electrode-based label-free electrochemical impedance spectroscopy immunosensor for sensitive detection of human malignant melanoma markers using gold nanoparticles functionalized magnetic graphene sheets as signal amplifier

机译：基于金纳米粒子功能化的石墨烯片作为信号放大器的基于磁性电极的无标记电化学阻抗光谱免疫传感器，用于敏感检测人恶性黑色素瘤标记物

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摘要

Cluster of differentiation 146 antigen (CD146) is a specific biomarker in the diagnosis of human malignant melanoma. In this work, a novel magnetic electrode-based label-free electrochemical impedance spectroscopy (EIS) immunosensor for quantitative detection of CD146 was established. Gold nanoparticles functionalized magnetic graphene sheets (Au@Fe3O4@GS) with good biocompatibility and electron transfer ability were used as transducing materials to achieve efficient conjugation of capture antibodies and signal amplification of the proposed immunosensor. For the facile and firm immobilization of magnetic transducing materials, the proposed immunosensor was fabricated on a magnetic glassy carbon electrode (MGCE). The effective immobilization of Au@Fe3O4@GS through the magnetic force of MGCE played a significant role in improving the stability and sensitivity of the proposed immunosensor. The EIS method was employed to record the impedance change of electrodes modified with different concentrations of CD146. The label-free EIS immunosensor exhibited a wide linear range from 5 pg mL(-1) to 500 ng mL(-1) with a low detection limit of 2.5 pg mL(-1) for CD146. The proposed immunosensor displayed an electrochemical performance with good reproducibility, selectivity and stability, which could provide potential applications in the clinical diagnosis of biomarkers.
机译：分化簇146抗原（CD146）是诊断人类恶性黑色素瘤的特异性生物标志物。在这项工作中，建立了一种新型的基于磁性电极的无标记电化学阻抗谱（EIS）免疫传感器，用于定量检测CD146。具有良好的生物相容性和电子转移能力的金纳米颗粒功能化的石墨烯片（Au @ Fe3O4 @ GS）被用作转导材料，以实现捕获抗体的有效缀合和拟议免疫传感器的信号放大。为了方便，牢固地固定磁转导材料，建议的免疫传感器是在磁性玻碳电极（MGCE）上制造的。通过MGCE的磁力有效地固定Au @ Fe3O4 @ GS在提高拟议免疫传感器的稳定性和灵敏度方面起着重要作用。采用EIS方法记录用不同浓度的CD146修饰的电极的阻抗变化。无标记的EIS免疫传感器的线性范围从5 pg mL（-1）到500 ng mL（-1），CD146的检测限低至2.5 pg mL（-1）。提出的免疫传感器具有良好的重现性，选择性和稳定性，具有电化学性能，可为生物标志物的临床诊断提供潜在的应用。

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